Partial structure and immunological properties of lipopolysaccharide from marine-derived Pseudomonas stutzeri KMM 226

The partial structure and immunology of the lipopolysaccharide (LPS) of Pseudomonas stutzeri KMM 226, a bacterium isolated from a seawater sample collected at a depth of 2000 m, was characterised. The O-polysaccharide was built up of disaccharide repeating units constituted by l -Rha p and d -Glc p...

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Bibliographic Details
Published in:	Antonie van Leeuwenhoek Vol. 110; no. 12; pp. 1569 - 1580
Main Authors:	Kokoulin, Maxim S., Sokolova, Ekaterina V., Elkin, Yuriy N., Romanenko, Lyudmila A., Mikhailov, Valery V., Komandrova, Nadezhda A.
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-12-2017 Springer Nature B.V
Subjects:	Biomedical and Life Sciences Chemical analysis Cytokines - blood Cytokines - metabolism E coli Fatty acids Humans Immunology Interleukin 10 Interleukin 6 Interleukin 8 Life Sciences Lipid A Lipid A - chemistry Lipid A - immunology Lipids Lipopolysaccharides Lipopolysaccharides - chemistry Lipopolysaccharides - immunology Magnetic Resonance Spectroscopy Marine biology Medical Microbiology Microbiology O Antigens - chemistry O Antigens - immunology Original Paper Plant Sciences Pseudomonas Pseudomonas Infections - blood Pseudomonas Infections - immunology Pseudomonas Infections - metabolism Pseudomonas stutzeri Pseudomonas stutzeri - classification Pseudomonas stutzeri - immunology Pseudomonas stutzeri - isolation & purification Samples Seawater Seawater - microbiology Soil Science & Conservation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Structural analysis Toxins Tumor necrosis factor-α Water analysis Water Microbiology Lipopolysaccharide Antagonist Polysaccharide Marine deep-sea environment Lipid A O-Polysaccharide Pseudomonas stutzeri
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Summary:	The partial structure and immunology of the lipopolysaccharide (LPS) of Pseudomonas stutzeri KMM 226, a bacterium isolated from a seawater sample collected at a depth of 2000 m, was characterised. The O-polysaccharide was built up of disaccharide repeating units constituted by l -Rha p and d -Glc p NAc: →2)-α- l -Rha p -(1→3)-α- d -Glc p NAc-(1→. The structural analysis of the lipid A showed a mixture of different species. The major species were hexa-acylated and penta-acylated lipids A, bearing the 12:0(3-OH) in amide linkage and 10:0(3-OH) in ester linkage, while the secondary fatty acids were present only as 12:0. The presence of 12:0(2-OH) was not detected. The immunology experiments demonstrated that P. stutzeri KMM 226 LPS displayed a low ability to induce TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine production and acted as an antagonist of hexa-acylated Escherichia coli LPS in human blood in vitro.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-6072 1572-9699
DOI:	10.1007/s10482-017-0907-6