In Vitro and in Vivo Comparative Study of Oral Nanoparticles and Gut Iontophoresis as Oral Delivery Systems for Insulin

In Vitro and in Vivo Comparative Study of Oral Nanoparticles and Gut Iontophoresis as Oral Delivery Systems for Insulin

Multiple daily injections of insulin for diabetes cause many hazards for diabetic patients. Oral noninvasive insulin delivery could be more convenient and less painful than parenteral route. In past decades transdermal iontophoresis had been studied for insulin delivery across the skin with or witho...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin Vol. 44; no. 2; pp. 251 - 258
Main Authors: Elkhatib, Mona M., Ali, Amir I., Al-badrawy, Ali S.
Format: Journal Article
Language:English
Published: Japan The Pharmaceutical Society of Japan 01-02-2021
Japan Science and Technology Agency
Subjects:
Blood glucose
Cell viability
Diabetes mellitus
Digestive system
Gastrointestinal tract
gut iontophoresis
Hypoglycemia
Insulin
Intestine
Iontophoresis
nanoparticle
Nanoparticles
oral delivery
nanoparticle
insulin
gut iontophoresis
oral delivery
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Summary:Multiple daily injections of insulin for diabetes cause many hazards for diabetic patients. Oral noninvasive insulin delivery could be more convenient and less painful than parenteral route. In past decades transdermal iontophoresis had been studied for insulin delivery across the skin with or without chemical permeation enhancers. However, the results of these studies were not efficacious and serum insulin levels were not therapeutically effective. In the present study an advanced technology “gut iontophoresis” for insulin delivery across the gut wall was compared with traditional oral insulin delivery in the form of nanoparticles. In vitro application of electric current to the intestinal membrane could enhance the flux of insulin nanoparticles (3.4 fold enhancement of insulin transport) from the donor to the receptor compartment in the Franz cell. In vivo iontophoresis of insulin nanoparticles through the gut wall would produce intense hypoglycemia (57% glycemia drop in 3 h) without damage of the intestinal tissues. Cell viability assay indicated that 50–500 µg/mL nanoparticles had no toxic effect on Caco-2 cells. Nanoparticles gut iontophoresis could be a promising non-invasive technique for oral insulin delivery.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b20-00737