Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt

Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed the plasma proteomic proﬁling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography magnetic beads (MB-HIC8) separation followed by Matrix assisted laser d...

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Bibliographic Details
Published in:	Asian Pacific journal of cancer prevention : APJCP Vol. 20; no. 1; pp. 175 - 184
Main Authors:	Zaki, Ahmad, Ramadan, Ragaa Abdelkader, Moez, Pacint, Ghareeb, Hala, Elkarmouty, Ahmad
Format:	Journal Article
Language:	English
Published:	Thailand West Asia Organization for Cancer Prevention 01-01-2019
Subjects:	Breast Neoplasms - blood Breast Neoplasms - metabolism Case-Control Studies Chemical Fractionation - methods Down-Regulation - physiology Egypt Female Humans Middle Aged Peptides - blood Proteome - metabolism Proteomics Sensitivity and Specificity Software Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Up-Regulation - physiology Egypt Matrix assisted laser desorption ionization/ time of flight mass spectrometry ClinProTools magnetic beads
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Summary:	Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed the plasma proteomic proﬁling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography magnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight mass spectrometry (MALDI-TOF MS). Results: ClinProTools software identiﬁed 92 peaks that differed among the analyzed groups, 33 peaks were signiﬁcantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks were down-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4 and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjects with 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of 100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishing BC patients from healthy controls.
ISSN:	1513-7368 2476-762X
DOI:	10.31557/APJCP.2019.20.1.175