Nigella sativa Extract Potentially Inhibited Methicillin Resistant Staphylococcus aureus Induced Infection in Rabbits: Potential Immunomodulatory and Growth Promoting Properties

Nigella sativa Extract Potentially Inhibited Methicillin Resistant Staphylococcus aureus Induced Infection in Rabbits: Potential Immunomodulatory and Growth Promoting Properties

Weaning is the most crucial period associated with increased stress and susceptibility to diseases in rabbits. Methicillin-resistant Staphylococcus aureus (MRSA), a historic emergent pathogen related to post weaning stressors, adversely affects rabbit’s growth rate and productive cycle. Since MRSA i...

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Bibliographic Details
Published in:Animals (Basel) Vol. 12; no. 19; p. 2635
Main Authors: Elmowalid, Gamal Abd Elmoneim, Ahmad, Adel Attia M., El-Hamid, Marwa I. Abd, Ibrahim, Doaa, Wahdan, Ali, El Oksh, Amal S. A., Yonis, Ahlam E., Elkady, Mohamed Abdelrazek, Ismail, Tamer Ahmed, Alkhedaide, Adel Qlayel, Elnahriry, Shimaa S.
Format: Journal Article
Language:English
Published: Basel MDPI AG 30-09-2022
MDPI
Subjects:
Antibacterial activity
Antibiotics
Antimicrobial agents
Antimicrobial resistance
antimicrobials
Bacteria
Clinical isolates
Experimental infection
Gram-positive bacteria
growth promotion
Growth rate
Health risks
Immune response
Immunomodulation
Immunostimulants
Laboratories
Methicillin
MRSA
Multidrug resistance
Natural products
Nigella sativa
Nigella sativa extract
Public health
Rabbits
Staphylococcus aureus
Staphylococcus infections
Weaning
United Kingdom > UK
England
Egypt
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Summary:Weaning is the most crucial period associated with increased stress and susceptibility to diseases in rabbits. Methicillin-resistant Staphylococcus aureus (MRSA), a historic emergent pathogen related to post weaning stressors, adversely affects rabbit’s growth rate and productive cycle. Since MRSA is rapidly evolving antibiotics resistance, natural products are desperately required to tackle the public health threats posed by antimicrobial resistance. Thus, this study aimed to screen the iin vitro antibacterial activity of Nigella sativa extract (NSE) and its interactions with antibiotics against MRSA isolates. Moreover, 200 weaned rabbits were divided into 4 groups to investigate the iin vivo superiority of NSE graded levels towards growth performance, tight junction integrity, immune responsiveness and resistance against MRSA. Herein, NSE showed promising antimicrobial activities against MRSA isolates from animal (77.8%) and human (64.3%) origins. Additionally, MRSA isolates exposed to NSE became sensitive to all antimicrobials to which they were previously resistant. Our results described that the growth-promoting functions of NSE, especially at higher levels, were supported by elevated activities of digestive linked enzymes. Post-NSE feeding, rabbits’ sera mediated bactericidal activities against MRSA. Notably, upregulated expression of occludin, CLDN-1, MUC-2 and JAM-2 genes was noted post NSE supplementation with maximum transcriptional levels in 500 mg/kg NSE fed group. Our data described that NSE constitutively motivated rabbits’ immune responses and protected them against MRSA-induced experimental infection. Our results suggest the antimicrobial, growth stimulating and immunomodulation activities of NSE to maximize the capability of rabbits for disease response.
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ISSN:2076-2615
2076-2615
DOI:10.3390/ani12192635