Search Results - "Elizabeth Villa"

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein by Lu, Shan, Ye, Qiaozhen, Singh, Digvijay, Cao, Yong, Diedrich, Jolene K., Yates, John R., Villa, Elizabeth, Cleveland, Don W., Corbett, Kevin D.

“…The multifunctional nucleocapsid (N) protein in SARS-CoV-2 binds the ~30 kb viral RNA genome to aid its packaging into the 80–90 nm membrane-enveloped virion…”
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The In Situ Structure of Parkinson’s Disease-Linked LRRK2 by Watanabe, Reika, Buschauer, Robert, Böhning, Jan, Audagnotto, Martina, Lasker, Keren, Lu, Tsan-Wen, Boassa, Daniela, Taylor, Susan, Villa, Elizabeth

“…Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson’s disease. LRRK2 is a multi-domain protein containing a…”
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Visualizing the molecular sociology at the HeLa cell nuclear periphery by Mahamid, Julia, Pfeffer, Stefan, Schaffer, Miroslava, Villa, Elizabeth, Danev, Radostin, Cuellar, Luis Kuhn, Förster, Friedrich, Hyman, Anthony A., Plitzko, Jürgen M., Baumeister, Wolfgang

“…The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo–electron tomography (cryo-ET)…”
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Finding the right fit: chiseling structures out of cryo-electron microscopy maps by Villa, Elizabeth, Lasker, Keren

“…•Cryo-EM produces structures of macromolecular complexes at resolutions between 3 and 20Å.•Fitting approaches to build atomic models vary depending on the…”
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Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach by Lasker, Keren, Förster, Friedrich, Bohn, Stefan, Walzthoeni, Thomas, Villa, Elizabeth, Unverdorben, Pia, Beck, Florian, Aebersold, Ruedi, Sali, Andrej, Baumeister, Wolfgang

“…The 26S proteasome is at the executive end of the ubiquitin-proteasome pathway for the controlled degradation of intracellular proteins. While the structure of…”
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Assembly of a nucleus-like structure during viral replication in bacteria by Chaikeeratisak, Vorrapon, Nguyen, Katrina, Khanna, Kanika, Brilot, Axel F., Erb, Marcella L., Coker, Joanna K. C., Vavilina, Anastasia, Newton, Gerald L., Buschauer, Robert, Pogliano, Kit, Villa, Elizabeth, Agard, David A., Pogliano, Joe

“…We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed…”
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The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems by Lasker, Keren, Boeynaems, Steven, Lam, Vinson, Scholl, Daniel, Stainton, Emma, Briner, Adam, Jacquemyn, Maarten, Daelemans, Dirk, Deniz, Ashok, Villa, Elizabeth, Holehouse, Alex S., Gitler, Aaron D., Shapiro, Lucy

“…Intracellular phase separation is emerging as a universal principle for organizing biochemical reactions in time and space. It remains incompletely resolved…”
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Acinetobacter baumannii Catabolizes Ethanolamine in the Absence of a Metabolosome and Converts Cobinamide into Adenosylated Cobamides by Villa, Elizabeth A., Escalante-Semerena, Jorge C.

“…Acinetobacter baumannii is an opportunistic pathogen typically associated with hospital-acquired infections. Our understanding of the metabolism and physiology…”
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Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography by Engel, Benjamin D, Schaffer, Miroslava, Kuhn Cuellar, Luis, Villa, Elizabeth, Plitzko, Jürgen M, Baumeister, Wolfgang

“…Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with…”
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Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography by Rigort, Alexander, Bäuerlein, Felix J. B, Villa, Elizabeth, Eibauer, Matthias, Laugks, Tim, Baumeister, Wolfgang, Plitzko, Jürgen M

“…Cryoelectron tomography provides unprecedented insights into the macromolecular and supramolecular organization of cells in a close-to-living state. However…”
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Near-atomic resolution structural model of the yeast 26S proteasome by Beck, Florian, Unverdorben, Pia, Bohn, Stefan, Schweitzer, Andreas, Pfeifer, Günter, Sakata, Eri, Nickell, Stephan, Plitzko, Jürgen M., Villa, Elizabeth, Baumeister, Wolfgang, Förster, Friedrich

“…The 26S proteasome operates at the executive end of the ubiquitinproteasome pathway. Here, we present a cryo-EM structure of the Saccharomyces cerevisiae 26S…”
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Molecular dynamics flexible fitting: A practical guide to combine cryo-electron microscopy and X-ray crystallography by Trabuco, Leonardo G., Villa, Elizabeth, Schreiner, Eduard, Harrison, Christopher B., Schulten, Klaus

“…Hybrid computational methods for combining structural data from different sources and resolutions are becoming an essential part of structural biology,…”
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Viral Capsid Trafficking along Treadmilling Tubulin Filaments in Bacteria by Chaikeeratisak, Vorrapon, Khanna, Kanika, Nguyen, Katrina T., Sugie, Joseph, Egan, MacKennon E., Erb, Marcella L., Vavilina, Anastasia, Nonejuie, Poochit, Nieweglowska, Eliza, Pogliano, Kit, Agard, David A., Villa, Elizabeth, Pogliano, Joe

“…Cargo trafficking along microtubules is exploited by eukaryotic viruses, but no such examples have been reported in bacteria. Several large Pseudomonas phages…”
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Asymmetric localization of the cell division machinery during Bacillus subtilis sporulation by Khanna, Kanika, Garrido, Javier Lopez, Sugie, Joseph, Pogliano, Kit, Villa, Elizabeth

“…The Gram-positive bacterium can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter…”
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Selective transport of fluorescent proteins into the phage nucleus by Nguyen, Katrina T, Sugie, Joseph, Khanna, Kanika, Egan, MacKennon E, Birkholz, Erica A, Lee, Jina, Beierschmitt, Christopher, Villa, Elizabeth, Pogliano, Joe

“…Upon infection of Pseudomonas cells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous…”
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Relative contributions of norspermidine synthesis and signaling pathways to the regulation of Vibrio cholerae biofilm formation by Wotanis, Caitlin K, Brennan, 3rd, William P, Angotti, Anthony D, Villa, Elizabeth A, Zayner, Josiah P, Mozina, Alexandra N, Rutkovsky, Alexandria C, Sobe, Richard C, Bond, Whitney G, Karatan, Ece

“…The polyamine norspermidine is one of the major polyamines synthesized by Vibrionales and has also been found in various aquatic organisms. Norspermidine is…”
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Regulation of the Protein-Conducting Channel by a Bound Ribosome by Gumbart, James, Trabuco, Leonardo G., Schreiner, Eduard, Villa, Elizabeth, Schulten, Klaus

“…During protein synthesis, it is often necessary for the ribosome to form a complex with a membrane-bound channel, the SecY/Sec61 complex, in order to…”
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Models in vivo and in vitro for the study of acute and chronic inflammatory activity: A comprehensive review by Bernardette Martínez-Rizo, Abril, Fosado-Rodríguez, Ricardo, César Torres-Romero, Julio, César Lara-Riegos, Julio, Alberto Ramírez-Camacho, Mario, Ly Arroyo Herrera, Ana, Elizabeth Villa de la Torre, Fabiola, Ceballos Góngora, Emanuel, Ermilo Arana-Argáez, Víctor

“…•Update acute inflammation models.•Overview of Valid Chronic Inflammation Models.•Advantages and drawbacks of in vitro models. Inflammatory conditions are…”
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Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution by Armache, Jean-Paul, Jarasch, Alexander, Anger, Andreas M, Villa, Elizabeth, Becker, Thomas, Bhushan, Shashi, Jossinet, Fabrice, Habeck, Michael, Dindar, Gülcin, Franckenberg, Sibylle, Marquez, Viter, Mielke, Thorsten, Thomm, Michael, Berninghausen, Otto, Beatrix, Birgitta, Söding, Johannes, Westhof, Eric, Wilson, Daniel N, Beckmann, Roland

“…Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures…”
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Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis by Villa, Elizabeth, Sengupta, Jayati, Trabuco, Leonardo G, LeBarron, Jamie, Baxter, William T, Shaikh, Tanvir R, Grassucci, Robert A, Nissen, Poul, Ehrenberg, Måns, Schulten, Klaus, Frank, Joachim

“…In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by…”
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