Does granulosa cell apoptosis have a role in the etiology of unexplained infertility?

Objective( s): To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Study design: Setting was IVF-ET Unit of Cerrahpasa Medical Faculty, İstanbul University. GnRH analogs and g...

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Bibliographic Details
Published in:	European journal of obstetrics & gynecology and reproductive biology Vol. 112; no. 2; pp. 182 - 184
Main Authors:	İdil, Mehmet, Çepni, İsmail, Demirsoy, Gülten, Öcal, Pelin, Salihoğlu, Funda, Şenol, Hülya, Elibol, Feyhan, İrez, Tülay
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 10-02-2004 Elsevier
Subjects:	Adult Apoptosis Apoptosis - physiology Biological and medical sciences Birth control Cells, Cultured Embryo Transfer Female Fertilization in Vitro - methods Follicle Stimulating Hormone - therapeutic use Gonadotropin-Releasing Hormone - therapeutic use Granulosa cell Granulosa Cells - pathology Gynecology. Andrology. Obstetrics Humans Infertility, Female - etiology Infertility, Female - therapy Medical sciences Ovulation Induction - methods Probability Prospective Studies Risk Factors Sensitivity and Specificity Statistics, Nonparametric Sterility. Assisted procreation Unexplained infertility Unexplained infertility Granulosa cell Apoptosis Etiology Gynecology Obstetrics Sterility
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Summary:	Objective( s): To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Study design: Setting was IVF-ET Unit of Cerrahpasa Medical Faculty, İstanbul University. GnRH analogs and gonadotropins were used for ovulation induction in patients with unexplained infertility ( n=15) and tubal factor ( n=15) undergoing in vitro fertilization and embryo transfer (IVF-ET) procedures. Following HCG injection and follicular aspiration, apoptosis of granulosa cells was assessed using the in situ DNA nick end labelling method and apoptosis rate was further determined by flow cytometry. Apoptosis rates were compared between two groups. Mann–Whitney’s U-test and Student’s t-test were used for statistics. Results: Apoptosis rate was significantly higher in the unexplained infertility group (33.20±35.62% versus 10.10±17.23%). Conclusion( s): Granulosa cell apoptosis seems to have a role in the etiology of unexplained infertility.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0301-2115 1872-7654
DOI:	10.1016/S0301-2115(03)00365-8