Annexin A2, autoimmunity, anxiety and depression

Abstract Objectives Antiphospholipid syndrome (APS) is associated with neurological manifestations and one of the novel autoantigens associated with this disease is Annexin A2 (ANXA2). In this work we have examined the effect of high levels of autoantibodies to ANXA2 on the brain in a mouse model. M...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of autoimmunity Vol. 73; pp. 92 - 99
Main Authors:	Weiss, R, Bitton, A, Ben Shimon, M, Elhaik Goldman, S, Nahary, L, Cooper, I, Benhar, I, Pick, C.G, Chapman, J
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-09-2016
Subjects:	Adjuvants, Immunologic - administration & dosage Adrenal Cortex Hormones - blood Allergy and Immunology Animals Annexin Annexin A2 - immunology Annexin A2 - metabolism Antiphospholipid syndrome Antiphospholipid Syndrome - immunology Antiphospholipid Syndrome - psychology Anxiety Anxiety - blood Anxiety - immunology Anxiety - pathology Autoantibodies - analysis Autoantibodies - immunology Autoantigens - immunology Autoimmunity Brain - pathology Depression Depression - blood Depression - immunology Depression - pathology Disease Models, Animal Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G - analysis Immunoglobulin G - immunology Mice Mice, Inbred BALB C P11 Protein Multimerization Psychological Tests Recombinant Proteins - immunology S100 Proteins - metabolism S100A10 Autoimmunity P11 Depression Anxiety Annexin S100A10 Antiphospholipid syndrome
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Abstract Objectives Antiphospholipid syndrome (APS) is associated with neurological manifestations and one of the novel autoantigens associated with this disease is Annexin A2 (ANXA2). In this work we have examined the effect of high levels of autoantibodies to ANXA2 on the brain in a mouse model. Methods Recombinant ANXA2 emulsified in adjuvant was used to immunize mice while mice immunized with adjuvant only served as controls. At peak antibody levels the animal underwent behavioral and cognitive tests and their brains were examined for ANXA2 immunoglobulin G (IgG) and expression of ANXA2 and the closely linked protein p11. Results Very high levels of anti-ANXA2 antibodies (Abs) were associated with reduced anxiety in the open field 13.14% ± 0.89% of the time in the center compared to 8.64% ± 0.91% observed in the control mice (p < 0.001 by t-test). A forced swim test found significantly less depression manifested by immobility in the ANXA2 group. The changes in behavior were accompanied by a significant reduction in serum corticosteroid levels of ANXA2 group compared to controls. Moreover, higher levels of total IgG and p11 expression were found in ANXA2 group brains. Lower levels of circulating anti-ANXA2 Abs were not associated with behavioral changes. Conclusions We have established an animal model with high levels of anti-ANXA2 Abs which induced IgG accumulation in the brain and specific anxiolytic and anti-depressive effects. This model promises to further our understanding of autoimmune disease such as APS and to provide better understanding of the role of the ANXA2-p11 complex in the brain.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0896-8411 1095-9157
DOI:	10.1016/j.jaut.2016.06.011