Genes involved in copper resistance influence survival of Pseudomonas aeruginosa on copper surfaces

Genes involved in copper resistance influence survival of Pseudomonas aeruginosa on copper surfaces

To evaluate the killing of Pseudomonas aeruginosa PAO1 on copper cast alloys and the influence of genes on survival on copper containing medium and surfaces. Different strains of P. aeruginosa were inoculated on copper containing medium or different copper cast alloys and the survival rate determine...

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Bibliographic Details
Published in:Journal of applied microbiology Vol. 106; no. 5; pp. 1448 - 1455
Main Authors: Elguindi, J, Wagner, J, Rensing, C
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-05-2009
Blackwell Publishing Ltd
Blackwell
Subjects:
Alloys
Biological and medical sciences
Copper - pharmacology
copper cast alloys
copper toxicity
Drug Resistance, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial - physiology
Genes, Bacterial
Microbial Sensitivity Tests
Microbiology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - growth & development
survival assay
Temperature
Time Factors
Pseudomonadales
Applied microbiology
Toxicity
Alloys
Survival
copper cast alloys
Resistance
Gene
survival assay
Bacteria
Pseudomonadaceae
Pseudomonas aeruginosa
copper toxicity
Copper
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Summary:To evaluate the killing of Pseudomonas aeruginosa PAO1 on copper cast alloys and the influence of genes on survival on copper containing medium and surfaces. Different strains of P. aeruginosa were inoculated on copper containing medium or different copper cast alloys and the survival rate determined. The survival rates were compared with rates on copper-free medium and stainless steel as control. In addition, the effect of temperature on survival was examined. Copper cast alloys had been previously shown to be bactericidal to various bacteria, but the mechanism of copper-mediated killing is still not known. In this report, we demonstrate that P. aeruginosa PAO1 is rapidly killed on different copper cast alloys and that genes involved in conferring copper resistance in copper-containing medium also influenced survival on copper cast alloys. We also show that the rate of killing is influenced by temperature. To use copper surfaces more widely as bactericidal agents in various settings, it is important to understand how genes influence survival on these surfaces. Here we show that genes shown to be involved in copper resistance in P. aeruginosa PAO1 can have an impact on the length of survival time on copper cast alloys under certain conditions. This is an important first step for evaluation of future use of copper surfaces as bactericidal agents.
Bibliography:http://dx.doi.org/10.1111/j.1365-2672.2009.04148.x
These authors contributed equally.
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SourceType-Scholarly Journals-1
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ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2009.04148.x