Detection of human enteric viruses in shellfish collected in Tunisia

Detection of human enteric viruses in shellfish collected in Tunisia

The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Viruses were eluted from digestive tissues and co...

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Bibliographic Details
Published in:Letters in applied microbiology Vol. 43; no. 4; pp. 399 - 404
Main Authors: Elamri, D.E, Aouni, M, Parnaudeau, S, Le Guyader, F.S
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-10-2006
Blackwell Publishing Ltd
Blackwell Science
Subjects:
Astrovirus
Biological and medical sciences
Enterovirus
Enterovirus - classification
Enterovirus - genetics
Enterovirus - isolation & purification
Fundamental and applied biological sciences. Psychology
Hepatitis A virus
human enteric viruses
Humans
Marine
Microbiology
Norovirus
Reverse Transcriptase Polymerase Chain Reaction
reverse transcription‐PCR
RNA, Viral - genetics
RNA, Viral - metabolism
shellfish
Shellfish - virology
Tunisia
Tunisia
Africa
Human
Polymerase chain reaction
Virus
Analysis method
reverse transcription- PCR
Shellfish
Applied microbiology
Reverse transcription
Detection
human enteric viruses
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Summary:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found positive for enterovirus. The mean number of positive samples found in this study is in accordance with the data found in the literature, indicating that no real difference exists in this respect among countries studied. A notable exception is HAV, which reflects the epidemiological status of the population. This study highlights the interest to analyse shellfish samples from different production areas. These data will be helpful to understand virus circulation and to improve shellfish safety. The results, which confirm contamination, necessitate the development of appropriate studies and monitoring in all shellfish-producing countries.
Bibliography:http://dx.doi.org/10.1111/j.1472-765X.2006.01978.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0266-8254
1472-765X
1365-2673
DOI:10.1111/j.1472-765X.2006.01978.x