Role of microRNA-21 and microRNA-155 as biomarkers for bronchial asthma

Role of microRNA-21 and microRNA-155 as biomarkers for bronchial asthma

MicroRNA (miRNA)-21 and miRNA-155 are important regulators of gene expression of different immunological molecules. This study aimed to investigate the role of miRNA-21 and miRNA-155 as biomarkers in asthma by comparing their serum expression levels in asthmatic patients to those in healthy controls...

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Bibliographic Details
Published in:Innate immunity (London, England) Vol. 27; no. 1; pp. 61 - 69
Main Authors: ElKashef, Sabrin Mohamed Mohamed Abd Elhamed, Ahmad, Sohair El-Attar, Soliman, Youssef Mohamed Amin, Mostafa, Marwa Salah
Format: Journal Article
Language:English
Published: London, England SAGE Publications 01-01-2021
SAGE PUBLICATIONS, INC
SAGE Publishing
Subjects:
Adult
Asthma
Asthma - genetics
Asthma - immunology
Biomarkers
Enzyme-linked immunosorbent assay
Female
Gene expression
Gene Expression Regulation - genetics
Humans
Immunity, Innate - genetics
Interleukin 4
Interleukin-4 - genetics
Leukocytes (eosinophilic)
Male
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Original
Polymerase chain reaction
Reference Values
ROC Curve
Sensitivity and Specificity
Serum levels
Smoking - adverse effects
Statistical analysis
IL-4
pathogenesis
miRNA-155
miRNA-21
Asthma
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Summary:MicroRNA (miRNA)-21 and miRNA-155 are important regulators of gene expression of different immunological molecules. This study aimed to investigate the role of miRNA-21 and miRNA-155 as biomarkers in asthma by comparing their serum expression levels in asthmatic patients to those in healthy controls and correlating their levels with serum IL-4. The expression levels of miRNA-21 and miRNA-155 were evaluated by quantitative RT-PCR. Serum levels of IL-4 were determined using ELISA. Asthmatic patients showed significantly higher serum miRNA-21 and miRNA-155 expression levels compared to controls. A statistically significant positive correlation between the expression levels of miRNA-21 and IL-4 serum levels in asthmatic patients was detected. Nonetheless, no correlation was detected between miRNA-155 expression and each of IL-4 and miRNA-21. A receiver operating characteristic curve analysis showed that at a cut-off value of 1.37, the sensitivity of miRNA-21 as an asthma biomarker was 100% and the specificity was 95%. At a cut-off value of 1.96, the sensitivity of miRNA-155 as an asthma biomarker was 100% and the specificity was 100%. It can be concluded that miRNA-21 and miRNA-155 are potential non-invasive biomarkers in the diagnosis of eosinophilic asthma and its response to therapy.
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ISSN:1753-4259
1753-4267
DOI:10.1177/1753425920901563