Study the relationship between long non-coding RNA (CCAT1) expression and CDK4 expression levels in Egyptian patients with preeclampsia
Maternal and perinatal mortality is caused by a variety of factors, including preeclampsia. PE's onset and progression may be influenced by lncRNAs. The effect of long non-coding RNA (lncRNA) Colon cancer-associated transcription factor 1 (CCAT1) expression in the placenta of preeclampsia patie...
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| Published in: | Biochemistry and biophysics reports Vol. 31; p. 101294 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Netherlands
Elsevier B.V
01-09-2022
Elsevier |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Maternal and perinatal mortality is caused by a variety of factors, including preeclampsia. PE's onset and progression may be influenced by lncRNAs. The effect of long non-coding RNA (lncRNA) Colon cancer-associated transcription factor 1 (CCAT1) expression in the placenta of preeclampsia patients on preeclampsia progression was the focus of this investigation.
The aim of the current study is to check if the levels of expression of Colon cancer-associated transcription factor 1 (CCAT1) and Cyclin-dependent protein kinase 4 (CDK4) are associated with preeclampsia vulnerability and biogenesis.
and methods: This work included the participation of 160 people. Eighty of the patients had preeclampsia. The control group included 80 normal pregnant women. The two groups were almost of the same age. A thorough clinical examination was performed in all groups (including taking a detailed history, concentrating on parity, age and previous background of diabetes or hypertension). The expression levels of CCAT1 and CDK4 in placental tissue were determined using a real-time q PCR technique.
Expression levels of CCAT1 and CDK4 differed significantly between the study groups. preeclamptic patients having the highest level of CCAT1in comparison with control group, However, preeclamptic patients having lower level of CDK4 than controls. There was a strong negative association between CDK4 expression level and DBP, SBP, creatinine, urea and CCAT1 level of expression in the preeclamptic group, whereas there was a positive correlation between CCAT1 level of expression and DBP, SBP, urea and creatinine in patients group.
Based on the findings of this study, it is possible that CCAT1 and CDK4 expression levels could be used to aid in the diagnosis and biogenesis of preeclampsia.
•Preeclampsia is a time-progressing multisystem disease a characteristic of the development of hypertension and proteinuria in late pregnancy or postpartum with severe organ malfunction.•Long non-coding RNAs have a major role in the improvement of the placental development and trophoblastic growth. lncRNAs play a variety of roles in cell biology (for example, stem cell transformation, cell differentiation, proliferation,and gene imprinting),•We discovered a substantial statistical difference between preeclampsia and control in terms of CAAT1 expression, with PE having the highest level of expression than the control group.•CCAT1 overexpression inhibits the cyclin D1-P16-CDK4 pathway, which reduces trophoblast proliferation.•This analysis showed that high CDK4 expression was a factor of protection against preeclampsia. This is because the levels significantly reduced in these patients in comparison with healthy group. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 2405-5808 2405-5808 |
| DOI: | 10.1016/j.bbrep.2022.101294 |