Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques

Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex ( Mtb c), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the as...

Full description

Saved in:

Bibliographic Details
Published in:	Scientific reports Vol. 14; no. 1; p. 3404
Main Authors:	Sawaswong, Vorthon, Chanchaem, Prangwalai, Klomkliew, Pavit, Rotcheewaphan, Suwatchareeporn, Meesawat, Suthirote, Kemthong, Taratorn, Kaewparuehaschai, Mutchamon, Noradechanon, Kirana, Ekatat, Monya, Kanitpun, Reka, Srilohasin, Prapaporn, Warit, Saradee, Chaiprasert, Angkana, Malaivijitnond, Suchinda, Payungporn, Sunchai
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 10-02-2024 Nature Publishing Group Nature Portfolio
Subjects:	631/326/2565/2134 631/601 Animals Cynomolgus macaque Full-length 16S sequencing Gastrointestinal Microbiome - genetics Gastrointestinal Microbiome - immunology Gut-pharyngeal microbiome Humanities and Social Sciences Immunoregulation Infectious diseases Macaca fascicularis - genetics Microbiomes Microbiota Microbiota - genetics multidisciplinary Nanopores Pharynx RNA, Ribosomal, 16S - genetics rRNA 16S Science Science (multidisciplinary) Tuberculosis Tuberculosis - microbiology Gut-pharyngeal microbiome Tuberculosis Full-length 16S sequencing Cynomolgus macaque
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex ( Mtb c), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (−) control, TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased, while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition, Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity, but details of mechanisms should be further explored and validated. In summary, microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction, which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-024-53880-w