Search Results - "Egelman, Edward H."

The Current Revolution in Cryo-EM by Egelman, Edward H.

“…Structural biology is the study of the molecular architecture of proteins and nucleic acids, which are the basis for all life forms. Structural biology came…”
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Unified Polymerization Mechanism for the Assembly of ASC-Dependent Inflammasomes by Lu, Alvin, Magupalli, Venkat Giri, Ruan, Jianbin, Yin, Qian, Atianand, Maninjay K., Vos, Matthijn R., Schröder, Gunnar F., Fitzgerald, Katherine A., Wu, Hao, Egelman, Edward H.

“…Inflammasomes elicit host defense inside cells by activating caspase-1 for cytokine maturation and cell death. AIM2 and NLRP3 are representative sensor…”
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Cryo-EM of bacterial pili and archaeal flagellar filaments by Egelman, Edward H

“…•Direct electron detectors allow for near-atomic resolution for many polymers.•Three types of bacterial pili discussed: chaperone-usher, mating, and Type…”
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Structural Architecture of the CARMA1/Bcl10/MALT1 Signalosome: Nucleation-Induced Filamentous Assembly by Qiao, Qi, Yang, Chenghua, Zheng, Chao, Fontán, Lorena, David, Liron, Yu, Xiong, Bracken, Clay, Rosen, Monica, Melnick, Ari, Egelman, Edward H., Wu, Hao

“…The CARMA1/Bcl10/MALT1 (CBM) signalosome mediates antigen receptor-induced NF-κB signaling to regulate multiple lymphocyte functions. While CARMA1 and Bcl10…”
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Structure of the Type VI Secretion System Contractile Sheath by Kudryashev, Mikhail, Wang, Ray Yu-Ruei, Brackmann, Maximilian, Scherer, Sebastian, Maier, Timm, Baker, David, DiMaio, Frank, Stahlberg, Henning, Egelman, Edward H., Basler, Marek

“…Bacteria use rapid contraction of a long sheath of the type VI secretion system (T6SS) to deliver effectors into a target cell. Here, we present an…”
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Molecular Imprinting as a Signal-Activation Mechanism of the Viral RNA Sensor RIG-I by Wu, Bin, Peisley, Alys, Tetrault, David, Li, Zongli, Egelman, Edward H., Magor, Katharine E., Walz, Thomas, Penczek, Pawel A., Hur, Sun

“…RIG-I activates interferon signaling pathways by promoting filament formation of the adaptor molecule, MAVS. Assembly of the MAVS filament is mediated by its…”
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Actin Filaments as Tension Sensors by Galkin, Vitold E., Orlova, Albina, Egelman, Edward H.

“…The field of mechanobiology has witnessed an explosive growth over the past several years as interest has greatly increased in understanding how mechanical…”
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Cryo-EM Structure of Caspase-8 Tandem DED Filament Reveals Assembly and Regulation Mechanisms of the Death-Inducing Signaling Complex by Fu, Tian-Min, Li, Yang, Lu, Alvin, Li, Zongli, Vajjhala, Parimala R., Cruz, Anthony C., Srivastava, Devendra B., DiMaio, Frank, Penczek, Pawel A., Siegel, Richard M., Stacey, Katryn J., Egelman, Edward H., Wu, Hao

“…Caspase-8 activation can be triggered by death receptor-mediated formation of the death-inducing signaling complex (DISC) and by the inflammasome adaptor ASC…”
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Structure of the Neisseria meningitidis Type IV pilus by Kolappan, Subramania, Coureuil, Mathieu, Yu, Xiong, Nassif, Xavier, Egelman, Edward H., Craig, Lisa

“…Neisseria meningitidis use Type IV pili (T4P) to adhere to endothelial cells and breach the blood brain barrier, causing cause fatal meningitis. T4P are…”
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Near-Atomic Resolution for One State of F-Actin by Galkin, Vitold E., Orlova, Albina, Vos, Matthijn R., Schröder, Gunnar F., Egelman, Edward H.

“…Actin functions as a helical polymer, F-actin, but attempts to build an atomic model for this filament have been hampered by the fact that the filament cannot…”
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Three-dimensional reconstruction of helical polymers by Egelman, Edward H.

“…•Helical protein and nucleoprotein polymers are ubiquitous in biology.•Three-dimensional reconstruction in electron microscopy began with helices.•Intrinsic…”
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Reconstruction of helical filaments and tubes by Egelman, Edward H

“…While Fourier-Bessel methods gave rise to the first three-dimensional reconstruction of an object from electron microscopic images, and these methods have…”
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Assembly-driven activation of the AIM2 foreign-dsDNA sensor provides a polymerization template for downstream ASC by Morrone, Seamus R., Matyszewski, Mariusz, Yu, Xiong, Delannoy, Michael, Egelman, Edward H., Sohn, Jungsan

“…AIM2 recognizes foreign dsDNA and assembles into the inflammasome, a filamentous supramolecular signalling platform required to launch innate immune responses…”
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Molecular mechanism for NLRP6 inflammasome assembly and activation by Shen, Chen, Lu, Alvin, Xie, Wen Jun, Ruan, Jianbin, Negro, Roberto, Egelman, Edward H., Fu, Tian-Min, Wu, Hao

“…Inflammasomes are large protein complexes that trigger host defense in cells by activating inflammatory caspases for cytokine maturation and pyroptosis. NLRP6…”
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Outcome of the First Electron Microscopy Validation Task Force Meeting by Henderson, Richard, Sali, Andrej, Baker, Matthew L., Carragher, Bridget, Devkota, Batsal, Downing, Kenneth H., Egelman, Edward H., Feng, Zukang, Frank, Joachim, Grigorieff, Nikolaus, Jiang, Wen, Ludtke, Steven J., Medalia, Ohad, Penczek, Pawel A., Rosenthal, Peter B., Rossmann, Michael G., Schmid, Michael F., Schröder, Gunnar F., Steven, Alasdair C., Stokes, David L., Westbrook, John D., Wriggers, Willy, Yang, Huanwang, Young, Jasmine, Berman, Helen M., Chiu, Wah, Kleywegt, Gerard J., Lawson, Catherine L.

“…This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Task Force organized by the…”
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Resolution advances in cryo-EM enable application to drug discovery by Subramaniam, Sriram, Earl, Lesley A, Falconieri, Veronica, Milne, Jacqueline LS, Egelman, Edward H

“…•There is rapid growth in number of structures determined using cryo-EM methods.•Single particle cryo-EM can now achieve resolutions better than 2Å.•Drug…”
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Type IV Pilus Structure by Cryo-Electron Microscopy and Crystallography: Implications for Pilus Assembly and Functions by Craig, Lisa, Volkmann, Niels, Arvai, Andrew S., Pique, Michael E., Yeager, Mark, Egelman, Edward H., Tainer, John A.

“…Type IV pili (T4P) are long, thin, flexible filaments on bacteria that undergo assembly-disassembly from inner membrane pilin subunits and exhibit astonishing…”
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Ambiguities in helical reconstruction by Egelman, Edward H

“…Helical polymers are found throughout biology and account for a substantial fraction of the protein in a cell. These filaments are very attractive for…”
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Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex by Costa, Tiago R.D., Ilangovan, Aravindan, Ukleja, Marta, Redzej, Adam, Santini, Joanne M., Smith, Terry K., Egelman, Edward H., Waksman, Gabriel

“…Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as…”
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A structural model of flagellar filament switching across multiple bacterial species by Wang, Fengbin, Burrage, Andrew M., Postel, Sandra, Clark, Reece E., Orlova, Albina, Sundberg, Eric J., Kearns, Daniel B., Egelman, Edward H.

“…The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states…”
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