Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains

Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains

Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among these brain cells. Current Brainbow based transgenic animals take the advantage of multispectral labeling to differentiate neighboring cells or l...

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Bibliographic Details
Published in:Frontiers in neural circuits Vol. 15; p. 732183
Main Authors: Li, Ye, Walker, Logan A, Zhao, Yimeng, Edwards, Erica M, Michki, Nigel S, Cheng, Hon Pong Jimmy, Ghazzi, Marya, Chen, Tiffany Y, Chen, Maggie, Roossien, Douglas H, Cai, Dawen
Format: Journal Article
Language:English
Published: Switzerland Frontiers Research Foundation 20-10-2021
Frontiers Media S.A
Subjects:
Animals
Animals, Genetically Modified
Axons
Bitbow
Brain
Brainbow
Cell differentiation
Codes
Color
Drosophila
Drosophila brain
Gene loci
Genomes
Image processing
Insects
Labeling
lineage tracing
Mapping
morphological analysis
Morphology
multicolor transgenics
Nervous system
Neural Circuits
Neural networks
Neurons
Transgenic animals
Bitbow
Drosophila brain
Brainbow
multicolor transgenics
lineage tracing
morphological analysis
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Summary:Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among these brain cells. Current Brainbow based transgenic animals take the advantage of multispectral labeling to differentiate neighboring cells or lineages, however, their applications are limited by the color capacity. To improve the analysis throughput, we designed Bitbow, a digital format of Brainbow which exponentially expands the color palette to provide tens of thousands of spectrally resolved unique labels. We generated transgenic Bitbow lines, established statistical tools, and streamlined sample preparation, image processing, and data analysis pipelines to conveniently mapping neural lineages, studying neuronal morphology and revealing neural network patterns with unprecedented speed, scale, and resolution.
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Reviewed by: Iris Salecker, INSERM U1024 Institut de Biologie de l’Ecole Normale Superieure, France; Gia Michele Ratto, National Research Council, Consiglio Nazionale delle Ricerche (CNR), Italy
Edited by: Junsong Zhang, Xiamen University, China
ISSN:1662-5110
1662-5110
DOI:10.3389/fncir.2021.732183