A hybrid technique for measurement of intra/extracellular proteins

ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid an...

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Bibliographic Details
Published in:	PloS one Vol. 18; no. 5; p. e0282948
Main Authors:	Edabashi, Haiam, Elghadafi, Radwa, Rajkanth, Nischwethaa, Mody, Janvi Saurabh, Ma, Weiyuan, Dibart, Serge, Tang, Xiaoren
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 04-05-2023 Public Library of Science (PLoS)
Subjects:	Analysis Antibodies Biology and Life Sciences Blotting, Western Engineering and Technology Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Evaluation Gene expression Measurement Medicine and Health Sciences Methods Physical Sciences Proteins Research and Analysis Methods Sensitivity and Specificity Variance analysis United States
Online Access:	Get full text
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Summary:	ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0282948