Search Results - "Eckhardt, Allen E"

Applications of electrowetting-based digital microfluidics in clinical diagnostics by Pollack, Michael G, Pamula, Vamsee K, Srinivasan, Vijay, Eckhardt, Allen E

“…Digital microfluidics based on electrowetting is a type of microfluidic platform in which liquids are processed as individual unit-sized droplets that are…”
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Fluorimetric assay with a novel substrate for quantification of galactocerebrosidase activity in dried blood spot specimens by Ullal, Anirudh J., Pham, Hong, Singh, Rajendra, Ross, Peter, Graham, Carrie A., Norton, Scott M., Nuffer, Miriam H., Burns, Debbie S., Eckhardt, Allen E., Escolar, Maria, Bali, Deeksha, Pamula, Vamsee K.

“…Decreased galactocerebrosidase (GALC) enzyme activity is causative for Krabbe disease, a lysosomal storage disorder with devastating neurodegenerative…”
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Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform by Sista, Ramakrishna S, Eckhardt, Allen E, Srinivasan, Vijay, Pollack, Michael G, Palanki, Srinivas, Pamula, Vamsee K

“…A digital microfluidic platform for performing heterogeneous sandwich immunoassays based on efficient handling of magnetic beads is presented in this paper…”
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Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform by Hua, Zhishan, Rouse, Jeremy L, Eckhardt, Allen E, Srinivasan, Vijay, Pamula, Vamsee K, Schell, Wiley A, Benton, Jonathan L, Mitchell, Thomas G, Pollack, Michael G

“…This paper details the development of a digital microfluidic platform for multiplexed real-time polymerase chain reactions (PCR). Liquid samples in discrete…”
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Digital Microfluidic Platform for Multiplexing Enzyme Assays: Implications for Lysosomal Storage Disease Screening in Newborns by SISTA, Ramakrishna S, ECKHARDT, Allen E, MILLINGTON, David S, PAMULA, Vamsee K, TONG WANG, GRAHAM, Carrie, ROUSE, Jeremy L, NORTON, Scott M, SRINIVASAN, Vijay, POLLACK, Michael G, TOLUN, Adviye A, BALI, Deeksha

“…Newborn screening for lysosomal storage diseases (LSDs) has been gaining considerable interest owing to the availability of enzyme replacement therapies. We…”
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Distinct β-Arrestin- and G Protein-dependent Pathways for Parathyroid Hormone Receptor-stimulated ERK1/2 Activation by Gesty-Palmer, Diane, Chen, Minyong, Reiter, Eric, Ahn, Seungkirl, Nelson, Christopher D., Wang, Shuntai, Eckhardt, Allen E., Cowan, Conrad L., Spurney, Robert F., Luttrell, Louis M., Lefkowitz, Robert J.

“…Parathyroid hormone (PTH) regulates calcium homeostasis via the type I PTH/PTH-related peptide (PTH/PTHrP) receptor (PTH1R). The purpose of the present study…”
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Droplet-Based Pyrosequencing Using Digital Microfluidics by Boles, Deborah J, Benton, Jonathan L, Siew, Germaine J, Levy, Miriam H, Thwar, Prasanna K, Sandahl, Melissa A, Rouse, Jeremy L, Perkins, Lisa C, Sudarsan, Arjun P, Jalili, Roxana, Pamula, Vamsee K, Srinivasan, Vijay, Fair, Richard B, Griffin, Peter B, Eckhardt, Allen E, Pollack, Michael G

“…The feasibility of implementing pyrosequencing chemistry within droplets using electrowetting-based digital microfluidics is reported. An array of electrodes…”
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Rapid, single-step assay for Hunter syndrome in dried blood spots using digital microfluidics by Sista, Ramakrishna, Eckhardt, Allen E., Wang, Tong, Séllos-Moura, Márcia, Pamula, Vamsee K.

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A novel fluorometric enzyme analysis method for Hunter syndrome using dried blood spots by Tolun, Adviye A., Graham, Carrie, Shi, Qun, Sista, Ramakrishna S., Wang, Tong, Eckhardt, Allen E., Pamula, Vamsee K., Millington, David S., Bali, Deeksha S.

“…Mucopolysaccharidosis type II (MPS II) or Hunter syndrome is a lysosomal storage disease caused by deficiency of iduronate-2-sulfatase (IDS). A convenient…”
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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens by Wulff-Burchfield, Elizabeth, Schell, Wiley A, Eckhardt, Allen E, Pollack, Michael G, Hua, Zhishan, Rouse, Jeremy L, Pamula, Vamsee K, Srinivasan, Vijay, Benton, Jonathan L, Alexander, Barbara D, Wilfret, David A, Kraft, Monica, Cairns, Charles B, Perfect, John R, Mitchell, Thomas G

“…Abstract Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and…”
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Probing Biomolecule Recognition with Electron Transfer:  Electrochemical Sensors for DNA Hybridization by Napier, Mary E, Loomis, Carson R, Sistare, Mark F, Kim, Jinheung, Eckhardt, Allen E, Thorp, H. Holden

“…Identifying infectious organisms, quantitating gene expression, and sequencing genomic DNA on chips all rely on the detection of nucleic acid hybridization…”
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Isolation and characterization of a family of .alpha.-D-galactosyl-containing glycopeptides from Ehrlich ascites tumor cells by Eckhardt, Allen E, Goldstein, Irwin J

“…A family of glycopeptides that contain nonreducing terminal alpha-D-galactosyl residues has been isolated from Pronase digests of delipidated Ehrlich ascites…”
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Porcine Submaxillary Mucin Forms Disulfide-linked Multimers through Its Amino-terminal D-domains by Perez-Vilar, Juan, Eckhardt, Allen E., DeLuca, Alexander, Hill, Robert L.

“…COS-7 cells expressing 1,360 residues from the amino terminus of porcine submaxillary mucin were used to determine whether this region, containing the D1, D2,…”
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Subcellular Localization of the UDP-N-Acetyl-D-Galactosamine: Polypeptide N-Acetylgalactosaminyltransferase-Mediated O-Glycosylation Reaction in the Submaxillary Gland by Roth, Jurgen, Wang, Yang, Eckhardt, Allen E., Hill, Robert L.

“…Addition of N-acetylgalactosamine to threonine and serine is the first step in the synthesis of O-glycosidically linked oligosaccharides. A…”
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Porcine Submaxillary Mucin Forms Disulfide-bonded Dimers between Its Carboxyl-terminal Domains (∗) by Perez-Vilar, Juan, Eckhardt, Allen E., Hill, Robert L.

“…COS-7 cells transfected with three different expression vectors encoding the 240-amino acid residue, disulfide-rich domain at the carboxyl terminus of porcine…”
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The Complete cDNA Sequence and Structural Polymorphism of the Polypeptide Chain of Porcine Submaxillary Mucin by Eckhardt, Allen E., Timpte, Candace S., DeLuca, Alexander W., Hill, Robert L.

“…The complete structure of the DNA encoding the polypeptide chain of porcine submaxillary mucin has been determined. The polypeptide is composed of distinct…”
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Occurrence of .alpha.-D-galactosyl-containing glycoproteins on Ehrlich tumor cell membranes by Eckhardt, Allen E, Goldstein, Irwin J

“…Ehrlich ascites tumor cells were strongly agglutinated by 0.4 micrograms/mL Griffonia simplicifolia I-B4 isolectin (GS I-B4), indicating the presence of…”
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Diagnostic value of a microfluidic based fluorometric enzyme assay platform using dried blood spots for a lysosomal disorder, Fabry disease by Tolun, Adviye A., Sista, Ramakrishna S., Eisenberg, Marcia, Eckhardt, Allen E., Pamula, Vamsee K., McDowell, Geraldine A.

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A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels by Eckhardt, A E, Hayes, C E, Goldstein, I J

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Inhibition of Ehrlich ascites tumor cell growth by Griffonia simplicifolia I lectin in vivo by Eckhardt, A E, Malone, B N, Goldstein, I J

“…Griffonia (Bandeiraea) simplicifolia I (GS I) seeds contain a family of alpha-D-galactopyranosyl-binding isolectins which strongly agglutinate Ehrlich ascites…”
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