Morphological and biological characterization of cell line developed from bovine Echinococcus granulosus

The taeniid tapeworm Echinococcus granulosus is the causative agent of echinococcal disease, a major zoonosis with worldwide distribution. Several efforts to establish an in vitro model of E. granulosus have been undertaken; however, many of them have been designed for Echinococcus multilocularis. I...

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Bibliographic Details
Published in:	In vitro cellular & developmental biology. Animal Vol. 46; no. 9; pp. 781 - 792
Main Authors:	Echeverría, Claudia I, Isolabella, Dora M, Gonzalez, Elio A. Prieto, Leonardelli, Araceli, Prada, Laura, Perrone, Alina, Fuchs, Alicia G
Format:	Journal Article
Language:	English
Published:	New York New York : Springer-Verlag 01-10-2010 Springer Science + Business Media Springer-Verlag Society for In Vitro Biology
Subjects:	Animal Genetics and Genomics Animals Antigens Artificial cells Biomedical and Life Sciences Cattle - parasitology Cell aggregates CELL AND TISSUE MODELS Cell Biology Cell Culture Cell culture techniques Cell growth Cell Line Cell lines Cell membranes Cell Proliferation Cultured cells Cysts Cytochromes Delta cells Developmental Biology DNA Primers - genetics Drugs Echinococcus granulosus Echinococcus granulosus - cytology Echinococcus multilocularis Electron Transport Complex IV - genetics Fluorescent Antibody Technique Growth factors Histological Techniques Karyotyping Life Sciences Microscopy Microscopy, Electron Mitochondria Nutrients Polymerase Chain Reaction Stem Cells Cyst formation Cell line characterization
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Summary:	The taeniid tapeworm Echinococcus granulosus is the causative agent of echinococcal disease, a major zoonosis with worldwide distribution. Several efforts to establish an in vitro model of E. granulosus have been undertaken; however, many of them have been designed for Echinococcus multilocularis. In the present study, we have described and characterized a stable cell line obtained from E. granulosus bovine protoscoleces maintained 3 yr in vitro. Growth characterization, morphology by light, fluorescent and electronic microscopy, and karyotyping were carried out. Cell culture origin was confirmed by immunofluorescent detection of AgB4 antigen and by PCR for the mitochondrial cytochrome c-oxidase subunit 1 (DCO1) gene. Cells seeded in agarose biphasic culture resembled a cystic structure, similar to the one formed in secondary hosts. This cell line could be a useful tool to research equinococcal behavior, allowing additional physiological and pharmacological studies, such as the effect of growth factors, nutrients, and antiparasitic drugs on cell viability and growth and on cyst formation.
Bibliography:	http://dx.doi.org/10.1007/s11626-010-9345-8 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	1071-2690 1543-706X
DOI:	10.1007/s11626-010-9345-8