Raman Spectroscopy for Rapid Evaluation of Surgical Margins during Breast Cancer Lumpectomy

Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on precisely distinguishing healthy from malignant tissue during surgery. Laser Raman spectroscopy (LRS) has been previously shown to differentiate be...

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Bibliographic Details
Published in:	Scientific reports Vol. 9; no. 1; pp. 14639 - 16
Main Authors:	Zúñiga, Willie C., Jones, Veronica, Anderson, Sarah M., Echevarria, Alex, Miller, Nathaniel L., Stashko, Connor, Schmolze, Daniel, Cha, Philip D., Kothari, Ragini, Fong, Yuman, Storrie-Lombardi, Michael C.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 10-10-2019 Nature Publishing Group Nature Portfolio
Subjects:	631/57 631/61 Benign Breast - pathology Breast - surgery Breast cancer Breast Neoplasms - diagnosis Breast Neoplasms - pathology Breast Neoplasms - surgery Female Humanities and Social Sciences Humans Intraoperative Care - economics Intraoperative Care - methods Lumpectomy Margins of Excision Mastectomy, Segmental multidisciplinary Principal Component Analysis Prognosis Raman spectroscopy Science Science (multidisciplinary) Spectrometers Spectroscopy Spectroscopy, Near-Infrared - instrumentation Spectroscopy, Near-Infrared - methods Spectrum analysis Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Statistical analysis Surgery Surgical outcomes Time Factors Tissues Wavelengths
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Summary:	Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on precisely distinguishing healthy from malignant tissue during surgery. Laser Raman spectroscopy (LRS) has been previously shown to differentiate benign from malignant tissue in real time. However, the cost, assembly effort, and technical expertise needed for construction and implementation of the technique have prohibited widespread adoption. Recently, Raman spectrometers have been developed for non-medical uses and have become commercially available and affordable. Here we demonstrate that this current generation of Raman spectrometers can readily identify cancer in breast surgical specimens. We evaluated two commercially available, portable, near-infrared Raman systems operating at excitation wavelengths of either 785 nm or 1064 nm, collecting a total of 164 Raman spectra from cancerous, benign, and transitional regions of resected breast tissue from six patients undergoing mastectomy. The spectra were classified using standard multivariate statistical techniques. We identified a minimal set of spectral bands sufficient to reliably distinguish between healthy and malignant tissue using either the 1064 nm or 785 nm system. Our results indicate that current generation Raman spectrometers can be used as a rapid diagnostic technique distinguishing benign from malignant tissue during surgery.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-019-51112-0