Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda with SMS reporting

Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda with SMS reporting

In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating the AMR crisis. To address this issue, this study aimed to adapt a one-step AMR testing protocol alongside an SMS (Short Mes...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 14; no. 1; p. 2730
Main Authors: Aruhomukama, Dickson, Magiidu, Walusimbi Talemwa, Katende, George, Ebwongu, Robert Innocent, Bulafu, Douglas, Kasolo, Rajab, Nakabuye, Hellen, Musoke, David, Asiimwe, Benon
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01-02-2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/326
692/700
Accuracy
Ampicillin
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Aztreonam
Bacteria
Cefotaxime
Ceftazidime
Ceftriaxone
Cefuroxime
Clinical microbiology
Communication
Drug resistance
E coli
Health sciences
Hospitals
Humanities and Social Sciences
Laboratories
Microbiology
multidisciplinary
Science
Science (multidisciplinary)
Sensitivity analysis
Sulfamethoxazole
Telemedicine
Trimethoprim
Uganda
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating the AMR crisis. To address this issue, this study aimed to adapt a one-step AMR testing protocol alongside an SMS (Short Message Service) result relay system (SRRS), with the potential to reduce the turnaround time for AMR testing and result communication from 4 days or more to 1 day in Ugandan clinical microbiology laboratories. Out of the 377 samples examined, 54 isolates were obtained. Notably, E. coli (61%) and K. pneumoniae (33%) were the most frequently identified, majority testing positive for ESBL. Evaluation of three AMR testing protocols revealed varying sensitivity and specificity, with Protocol A (ChromID ESBL-based) demonstrating high sensitivity (100%) but no calculable specificity, Protocol B (ceftazidime-based) showing high sensitivity (100%) and relatively low specificity (7.1%), and Protocol C (cefotaxime-based) exhibiting high sensitivity (97.8%) but no calculable specificity. ESBL positivity strongly correlated with resistance to specific antibiotics, including cefotaxime, ampicillin, and aztreonam (100%), cefuroxime (96%), ceftriaxone (93%), and trimethoprim sulfamethoxazole (87%). The potential of integrating an SRRS underscored the crucial role this could have in enabling efficient healthcare communication in AMR management. This study underscores the substantial potential of the tested protocols for accurately detecting ESBL production in clinical samples, potentially, providing a critical foundation for predicting and reporting AMR patterns. Although considerations related to specificity warrant careful assessment before widespread clinical adoption.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-53230-w