Cloning and Characterization of Mammalian Homologs of the Drosophila dunce+Gene

A probe representing the Drosophila dunce+(dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+gene were isolated from ra...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 86; no. 10; pp. 3604 - 3608
Main Authors:	Davis, Ronald L., Takayasu, Hiroko, Eberwine, Mary, Myres, James
Format:	Journal Article
Language:	English
Published:	Washington, DC National Academy of Sciences of the United States of America 01-05-1989 National Acad Sciences
Subjects:	3',5'-Cyclic-AMP Phosphodiesterases - genetics Amino Acid Sequence Amino acids Animals Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular Complementary DNA DNA DNA - genetics Drosophila Drosophila melanogaster - enzymology Drosophila melanogaster - genetics Enzymes Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes Genes. Genome Genomics Memory - physiology Molecular and cellular biology Molecular genetics Molecular Sequence Data Open reading frames Rats RNA Sequence Homology, Nucleic Acid Species Specificity Tissue Distribution Rat Insecta Homology Characterization Proteins Messenger RNA Gene Molecular cloning Southern blotting Ungulata Nucleic acid Aminoacid sequence Bovine Nucleotide sequence Gel electrophoresis Enzyme Rodentia Sequence alignment Cyclic AMP Gene expression Drosophilidae Northern blotting Vertebrata Mammalia Arthropoda Artiodactyla Invertebrata Drosophila melanogaster Diptera Comparative study
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Summary:	A probe representing the Drosophila dunce+(dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+gene were isolated from rat libraries and characterized. This gene has been named ratdnc-1. One cDNA clone defines a large open reading frame of ≈ 1.8 kilobases (kb), predicting a protein sequence of 610 amino acids with significant homology to a conserved domain of ≈ 275 residues found in most other PDEases. The amino acid identity value to the Drosophila cAMP PDEase within this domain is a striking 75%. Other cDNA clones show blocks of sequence divergence from this cDNA clone close to the predicted N terminus, indicating the potential existence of a family of related enzymes encoded by alternatively spliced messenger RNAs from ratdnc-1. Genomic blotting experiments suggest the existence of at least one other rat gene with homology to ratdnc-1. RNAs homologous to ratdnc-1 are heterogeneous in size between tissues, with heart containing a major transcript of 4.4 kb and brain one of 4.0 kb. The potential identity of the product of the ratdnc-1 gene with known PDEases is discussed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.86.10.3604