Search Results - "Ealick, Steven E"

Flavogenomics – a genomic and structural view of flavin‐dependent proteins by Macheroux, Peter, Kappes, Barbara, Ealick, Steven E.

“…Riboflavin (vitamin B2) serves as the precursor for FMN and FAD in almost all organisms that utilize the redox‐active isoalloxazine ring system as a coenzyme…”
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Structural studies of viperin, an antiviral radical SAM enzyme by Fenwick, Michael K., Li, Yue, Cresswell, Peter, Modis, Yorgo, Ealick, Steven E.

“…Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically…”
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The structural and biochemical foundations of thiamin biosynthesis by Jurgenson, Christopher T, Begley, Tadhg P, Ealick, Steven E

“…Thiamin is synthesized by most prokaryotes and by eukaryotes such as yeast and plants. In all cases, the thiazole and pyrimidine moieties are synthesized in…”
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Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase by Chatterjee, Abhishek, Abeydeera, N. Dinuka, Bale, Shridhar, Pai, Pei-Jing, Dorrestein, Pieter C., Russell, David H., Ealick, Steven E., Begley, Tadhg P.

“…Suicidal enzyme involved in vitamin B1 synthesis Vitamin B1 (thiamin) was the first vitamin to be discovered more than a century ago, but some of the details…”
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Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis by Dong, Min, Kathiresan, Venkatesan, Fenwick, Michael K, Torelli, Andrew T, Zhang, Yang, Caranto, Jonathan D, Dzikovski, Boris, Sharma, Ajay, Lancaster, Kyle M, Freed, Jack H, Ealick, Steven E, Hoffman, Brian M, Lin, Hening

“…Diphthamide biosynthesis involves a carbon-carbon bond-forming reaction catalyzed by a radical S-adenosylmethionine (SAM) enzyme that cleaves a carbon-sulfur…”
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Pyridoxal phosphate: Biosynthesis and catabolism by Mukherjee, Tathagata, Hanes, Jeremiah, Tews, Ivo, Ealick, Steven E., Begley, Tadhg P.

“…Vitamin B 6 is an essential cofactor that participates in a large number of biochemical reactions. Pyridoxal phosphate is biosynthesized de novo by two…”
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Thiamin biosynthesis: still yielding fascinating biological chemistry by Begley, Tadhg P, Ealick, Steven E, McLafferty, Fred W

“…The present paper describes the biosynthesis of the thiamin thiazole in Bacillus subtilis and Saccharomyces cerevisiae. The two pathways are quite different:…”
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Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily by Begley, Tadhg P, Booker, Squire J, Lee, Michael, Chatterjee, Abhishek, Li, Yue, Ealick, Steven E, Zhang, Yang, Grove, Tyler L, Krebs, Carsten

“…4-Amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase catalyzes a complex rearrangement of 5-aminoimidazole ribonucleotide (AIR) to form HMP-P,…”
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Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase by Fenwick, Michael K., Mehta, Angad P., Zhang, Yang, Abdelwahed, Sameh H., Begley, Tadhg P., Ealick, Steven E.

“…Radical S -adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5′-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a…”
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Different polyamine pathways from bacteria have replaced eukaryotic spermidine biosynthesis in ciliates Tetrahymena thermophila and Paramecium tetaurelia by Li, Bin, Kim, Sok Ho, Zhang, Yang, Hanfrey, Colin C., Elliott, Katherine A., Ealick, Steven E., Michael, Anthony J.

“…Summary The polyamine spermidine is absolutely required for growth and cell proliferation in eukaryotes, due to its role in post‐translational modification of…”
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Thiamin Pyrimidine Biosynthesis in Candida albicans: A Remarkable Reaction between Histidine and Pyridoxal Phosphate by Lai, Rung-Yi, Huang, Siyu, Fenwick, Michael K, Hazra, Amrita, Zhang, Yang, Rajashankar, Kanagalaghatta, Philmus, Benjamin, Kinsland, Cynthia, Sanders, Jennie Mansell, Ealick, Steven E, Begley, Tadhg P

“…In Saccharomyces cerevisiae, thiamin pyrimidine is formed from histidine and pyridoxal phosphate (PLP). The origin of all of the pyrimidine atoms has been…”
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Crystal Structure and Mechanism of Tryptophan 2,3-Dioxygenase, a Heme Enzyme Involved in Tryptophan Catabolism and in Quinolinate Biosynthesis by Zhang, Yang, Kang, Seong A, Mukherjee, Tathagata, Bale, Shridhar, Crane, Brian R, Begley, Tadhg P, Ealick, Steven E

“…The structure of tryptophan 2,3-dioxygenase (TDO) from Ralstonia metallidurans was determined at 2.4 Å. TDO catalyzes the irreversible oxidation of…”
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Catalysis of a Flavoenzyme-Mediated Amide Hydrolysis by Mukherjee, Tathagata, Zhang, Yang, Abdelwahed, Sameh, Ealick, Steven E, Begley, Tadhg P

“…A new pyrimidine catabolic pathway (the Rut pathway) was recently discovered in Escherichia coli K12. In this pathway, uracil is converted to…”
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Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites by Ye, Wenjie, Paul, Debamita, Gao, Lina, Seckute, Jolita, Sangaiah, Ramiah, Jayaraj, Karupiah, Zhang, Zhenfa, Kaminski, P Alexandre, Ealick, Steven E, Gold, Avram, Ball, Louise M

“…Deoxyribosyl transferases and functionally related purine nucleoside phosphorylases are used extensively for synthesis of non-natural deoxynucleosides as…”
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Biosynthesis of Thiamin Thiazole in Eukaryotes:  Conversion of NAD to an Advanced Intermediate by Chatterjee, Abhishek, Jurgenson, Christopher T, Schroeder, Frank C, Ealick, Steven E, Begley, Tadhg P

“…Thiazole synthase catalyzes the formation of the thiazole moiety of thiamin pyrophosphate. The enzyme from Saccharomyces cerevisiae (THI4) copurifies with a…”
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Structure of the PLP Degradative Enzyme 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase from Mesorhizobium loti MAFF303099 and Its Mechanistic Implications by McCulloch, Kathryn M, Mukherjee, Tathagata, Begley, Tadhg P, Ealick, Steven E

“…A vitamin B6 degradative pathway has recently been identified and characterized in Mesorhizobium loti MAFF303099. One of the enzymes on this pathway,…”
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Domain Organization in Candida glabrata THI6, a Bifunctional Enzyme Required for Thiamin Biosynthesis in Eukaryotes by Paul, Debamita, Chatterjee, Abhishek, Begley, Tadhg P, Ealick, Steven E

“…THI6 is a bifunctional enzyme found in the thiamin biosynthetic pathway in eukaryotes. The N-terminal domain of THI6 catalyzes the ligation of the thiamin…”
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Structure of Oxalate Decarboxylase from Bacillus subtilis at 1.75 Å Resolution by Anand, Ruchi, Dorrestein, Pieter C, Kinsland, Cynthia, Begley, Tadhg P, Ealick, Steven E

“…Oxalate decarboxylase is a manganese-dependent enzyme that catalyzes the conversion of oxalate to formate and carbon dioxide. We have determined the structure…”
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Structural Studies on 3-Hydroxyanthranilate-3,4-dioxygenase:  The Catalytic Mechanism of a Complex Oxidation Involved in NAD Biosynthesis by Zhang, Yang, Colabroy, Keri L, Begley, Tadhg P, Ealick, Steven E

“…3-Hydroxyanthranilate-3,4-dioxygenase (HAD) catalyzes the oxidative ring opening of 3-hydroxyanthranilate in the final enzymatic step of the biosynthetic…”
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Structural biology of enzymes of the thiamin biosynthesis pathway by Settembre, Ethan, Begley, Tadhg P, Ealick, Steven E

“…Thiamin pyrophosphate is an essential cofactor of carbohydrate and branched-chain amino acid metabolism. Although its mechanistic role is well studied, the…”
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