Search Results - "EGLEN, Richard"

Three-Dimensional Cell Cultures in Drug Discovery and Development by Fang, Ye, Eglen, Richard M

“…The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures as systems that better mimic in vivo…”
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Overview of muscarinic receptor subtypes by Eglen, Richard M

“…The physiological role of muscarinic receptors is highly complex and, although not completely understood, has become clearer over the last decade. Recent…”
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Muscarinic acetylcholine receptors: mutant mice provide new insights for drug development by Wess, Jürgen, Eglen, Richard M, Gautam, Dinesh

“…Muscarinic acetylcholine receptors (mAChRs), M(1)-M(5), regulate the activity of numerous fundamental central and peripheral functions. The lack of…”
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Drug Discovery Goes Three-Dimensional: Goodbye to Flat High-Throughput Screening? by Eglen, Richard M, Randle, David H

“…Immortalized cells, generated from two-dimensional cell culture techniques, are widely used in compound screening, lead optimization, and drug candidate…”
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Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 2: Spheroids, Organoids, and Disease Modeling by Eglen, Richard M, Reisine, Terry

“…Human induced pluripotent stem cells (HiPSCs) provide several advantages for drug discovery, but principally they provide a source of clinically relevant…”
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Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 1: Target Identification and Lead Optimization by Eglen, Richard M, Reisine, Terry

“…Human-induced pluripotent stem cells (HiPSCs), and new technologies to culture them into functional cell types and tissues, are now aiding drug discovery…”
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Screening for compounds that modulate epigenetic regulation of the transcriptome: an overview by Eglen, Richard M, Reisine, Terry

“…Epigenetic control of the transciptome is a complex and highly coordinated cellular process. One critical mechanism involves DNA methylation, mediated by…”
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Muscarinic receptor subtype pharmacology and physiology by Eglen, Richard M

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The current status of drug discovery against the human kinome by Eglen, Richard M, Reisine, Terry

“…Protein kinases are important targets in drug discovery programs aimed at treating many devastating diseases, including cancer, autoimmune disorders, diabetes,…”
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New insights into GPCR function: implications for HTS by Eglen, Richard M, Reisine, Terry

“…G protein-coupled receptors (GPCRs) are a large family of proteins that represent targets for approximately 40% of all approved drugs. They possess unique…”
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The use of immortalized cell lines in GPCR screening: the good, bad and ugly by Eglen, Richard M, Gilchrist, Annette, Reisine, Terry

“…For most membrane-bound molecular targets, including G protein linked receptors (GPCRs), the optimal approach in drug discovery involves the use of cell based…”
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Emerging concepts of guanine nucleotide-binding protein-coupled receptor (GPCR) function and implications for high throughput screening by Eglen, Richard M, Bosse, Roger, Reisine, Terry

“…Guanine nucleotide binding protein (G protein) coupled receptors (GPCRs) comprise one of the largest families of proteins in the human genome and are a target…”
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An overview of drug screening using primary and embryonic stem cells by Eglen, Richard M, Gilchrist, Annette, Reisine, Terry

“…Cellular technologies are widely used in drug discovery to treat human diseases. Most studies involve the expression of recombinant targets in immortalized…”
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Three-Dimensional Cell Culture: A Rapidly Emerging Approach to Cellular Science and Drug Discovery by Eglen, Richard M, Klein, Jean-Louis

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Functional G protein-coupled receptor assays for primary and secondary screening by Eglen, Richard M

“…Screening technologies for G protein-coupled receptors comprise two major approaches; homogeneous assays, conducted in microtiter plate formats, and protein…”
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GPCRs Revisited: New Insights Lead to Novel Drugs by Eglen, Richard M., Reisine, Terry

“…GPCRs play a critical role in human physiology and are a prime target for drug discovery globally. Novel insights into the functions of GPCRs are providing…”
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A generic, homogenous method for measuring kinase and inhibitor activity via adenosine 5'-diphosphate accumulation by Charter, Neil W, Kauffman, Lindy, Singh, Raj, Eglen, Richard M

“…The authors describe an assay to measure the generation of adenosine 5'-diphosphate (ADP) resulting from phosphorylation of a substrate by a kinase. ADP…”
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Distribution of the Tetrodotoxin-Resistant Sodium Channel PN3 in Rat Sensory Neurons in Normal and Neuropathic Conditions by Novakovic, Sanja D, Tzoumaka, Elda, McGivern, Joseph G, Haraguchi, Miki, Sangameswaran, Lakshmi, Gogas, Kathleen R, Eglen, Richard M, Hunter, John C

“…The novel sodium channel PN3/alpha-SNS, which was cloned from a rat dorsal root ganglion (DRG) cDNA library, is expressed predominantly in small sensory…”
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Beta galactosidase complementation: a cell-based luminescent assay platform for drug discovery by Olson, Keith R, Eglen, Richard M

“…Many cell-based assays interrogating cell pathway activation employ protocols that require microscopic imaging techniques. However, such assays are not in…”
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Photoproteins: important new tools in drug discovery by Eglen, Richard M, Reisine, Terry

“…The G protein-coupled receptor (GPCR) family is a major target for drug discovery, and most, if not all, GPCRs can couple to Ca2+ signaling. Consequently,…”
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