Comparative Analysis of Cells with Combined Apoptosis and Proliferation Markers in Thyroid Tissue Specimens from Patients with Cancer, Adenoma, and Autoimmune Diseases

Comparative Analysis of Cells with Combined Apoptosis and Proliferation Markers in Thyroid Tissue Specimens from Patients with Cancer, Adenoma, and Autoimmune Diseases

Combined phenotypes of cells with membrane and intracellular expression of apoptosis and proliferation regulation markers (p53, bcl-2, CD95, CD95L, Ki-67) were studied by flow cytometry of cell suspension from thyroid tissue specimens from patients with autoimmune diseases, adenoma, and thyroid canc...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine Vol. 150; no. 4; pp. 453 - 458
Main Authors: Kazakov, S. P., Zabotina, T. N., Korotkova, O. V., Dzabarov, A. K., Kazantseva, I. A.
Format: Journal Article
Language:English
Published: Boston Springer US 01-02-2011
Springer
Springer Nature B.V
Subjects:
Adenoma
Adenoma - diagnosis
Adenoma - metabolism
Adenoma - pathology
Adult
Aged
Apoptosis
Apoptosis - physiology
Autoimmune diseases
Autoimmune Diseases - diagnosis
Autoimmune Diseases - metabolism
Autoimmune Diseases - pathology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Proliferation
Fas Ligand Protein - metabolism
fas Receptor - metabolism
Female
Humans
Internal Medicine
Ki-67 Antigen - metabolism
Laboratory Medicine
Male
Middle Aged
Pathology
Proto-Oncogene Proteins c-bcl-2 - metabolism
Thyroid cancer
Thyroid Gland - cytology
Thyroid Gland - pathology
Thyroid Neoplasms - diagnosis
Thyroid Neoplasms - metabolism
Thyroid Neoplasms - pathology
Tumor proteins
Tumor Suppressor Protein p53 - metabolism
CD95L
Ki-67
CD95
thyroid cancer
bcl-2
p53
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Summary:Combined phenotypes of cells with membrane and intracellular expression of apoptosis and proliferation regulation markers (p53, bcl-2, CD95, CD95L, Ki-67) were studied by flow cytometry of cell suspension from thyroid tissue specimens from patients with autoimmune diseases, adenoma, and thyroid cancer. The incidence of cell groups with phenotypes p53/Ki-67, p53/CD95, bcl-2/Ki-67, bcl-2/CD95, CD95/Ki-67, p53/CD95L, CD95/CD95L, and bcl-2/CD95L was evaluated and the density of receptor distribution on/in each cell group are presented. Patients with autoimmune diseases had high incidence of cells with phenotypes p53/Ki-67, p53/CD95, bcl-2/Ki-67, bcl-2/CD95, CD95/Ki-67, p53/CD95L, CD95/CD95L, and bcl-2/CD95L; cells with the bcl-2/CD95 phenotype were the most incident. Patients with thyroid adenoma had high levels of cells with p53/CD95L phenotype, while patients with thyroid cancer had significantly lower levels of p53 expression in the p53/CD95L cell group. The density of CD95L receptors on CD95/CD95L-positive cells was 4-7-fold higher in patients with thyroid tumors; the density of CD95L receptors on CD95/CD95L cells was maximum in thyroid adenoma and minimum in thyroid cancer. These data indicate differences in the expression of apoptosis and proliferation markers in thyroid adenoma, cancer, and autoimmune diseases. Analysis of the expression of these markers in the above diseases can be useful for differential diagnosis.
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ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-011-1167-5