Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults

Vitamin D insufficiency, defined as 25-hydroxyvitamin D (25(OH)D) levels < 75nmol/L is associated with cardio-metabolic dysfunction. Vitamin D insufficiency is associated with inflammation and fibrosis, but it remains uncertain whether these anomalies are readily reversible. Therefore, we aimed t...

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Bibliographic Details
Published in:	PloS one Vol. 12; no. 5; p. e0174435
Main Authors:	Amarasekera, Anjalee T, Assadi-Khansari, Bahador, Liu, Saifei, Black, Marilyn, Dymmott, Greer, Rogers, Natasha M, Sverdlov, Aaron L, Horowitz, John D, Ngo, Doan T M
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 10-05-2017 Public Library of Science (PLoS)
Subjects:	25-Hydroxyvitamin D Activin Adult Biochemistry Biology and Life Sciences Biomarkers Blood Blood platelets Blood Pressure Calciferol Cardiology Cardiovascular disease Coronary vessels Diabetes Fasting Female Fibrosis Follistatin Gene expression Glycoproteins Growth factors Health aspects Healthy Volunteers Hospitals Humans Hypertension Insulin Lipids Male Medicine Medicine and Health Sciences Middle Aged Nitric oxide Pathogenesis Patients Pharmacy Physical sciences Physiology Plasminogen activator inhibitors Pressure effects Research and Analysis Methods Smooth muscle South Australia Studies Supplements Thrombospondin Thrombospondin 1 - blood Vitamin D Vitamin D - administration & dosage Vitamin deficiency Australia South Australia Australia
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Summary:	Vitamin D insufficiency, defined as 25-hydroxyvitamin D (25(OH)D) levels < 75nmol/L is associated with cardio-metabolic dysfunction. Vitamin D insufficiency is associated with inflammation and fibrosis, but it remains uncertain whether these anomalies are readily reversible. Therefore, we aimed to determine the effects of vitamin D supplementation on markers of: 1) nitric oxide (NO) signaling, 2) inflammation, and 3) fibrosis, in healthy volunteers with mild hypovitaminosis. Healthy volunteers (n = 35) (mean age: 45 ± 11 years) with 25(OH)D levels <75nmol/L, received vitamin D supplementation (Ostelin ® capsules 2000IU) for 12 weeks. Resting systolic and diastolic blood pressures (BP) were assessed. Routine biochemistry was examined. Plasma concentrations of asymmetric dimethylarginine (ADMA), thrombospondin-1 (TSP-1), plasminogen activator inhibitor-1 (PAI-1), hs-CRP, activin-A, and follistatin-like 3 (FSTL3) were quantitated. Vitamin D administration for 12 weeks significantly increased 25-(OH)D levels (48.8 ± 16 nmol/L to 100.8 ± 23.7 nmol/L, p<0.001). There was significant lowering of systolic and diastolic BP, while there was no significant change in lipid profiles, or fasting insulin. Plasma concentrations of ADMA, hs-CRP, PAI-1, activin A, and FSTL-3 did not change with vitamin D supplementation. However, there was a marked reduction of TSP-1 (522.7 ± 379.8 ng/mL vs 206.7 ± 204.5 ng/mL, p<0.001). Vitamin D supplementation in vitamin D insufficient, but otherwise healthy individuals markedly decreased TSP-1 levels and blood pressure. Since TSP-1 suppresses signaling of NO, it is possible that the fall in BP is engendered by restoration of NO effect.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceptualization: DN JH AS NR.Data curation: AA BA-K SL MB GD.Formal analysis: AA BA-K SL MB GD DN.Funding acquisition: DN AS.Investigation: AA BA-K SL MB GD.Methodology: AA BA-K SL MB GD.Project administration: DN.Resources: DN.Supervision: DN JH AS.Validation: AA BA-K SL MB GD NR JH AS DN.Writing – review & editing: AA BA-K SL MB GD NR JH AS DN. Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0174435