DNA Strand breaks in testicular cells from humans and rats following in vitro exposure to 1,2-dibromo-3-chloropropane (DBCP)

Preparations of testicular cells from human organ transplant donors and from Wistar rats were compared with respect to their composition of the different testicular cell types, their ability to metabolize 1,2-dibromo-3-chloropropane (DBCP), and their relative sensitivity to induction of DNA single s...

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Published in:	Reproductive toxicology (Elmsford, N.Y.) Vol. 10; no. 1; pp. 51 - 59
Main Authors:	Bjørge, Christine, Wiger, Richard, Holme, Jørn A., Brunborg, Gunnar, Scholz, Tim, Dyeing, Erik, Søderlund, Erik J.
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 1996 Elsevier
Subjects:	4-Nitroquinoline-1-oxide - toxicity 4-NQO Adult Aged Animals Binding Sites Biological and medical sciences Biotransformation Carcinogens - toxicity DBCP DNA Damage Electrophoresis Flow Cytometry Germ Cells - cytology Germ Cells - drug effects Germ Cells - radiation effects Germ Cells - ultrastructure human Humans In Vitro Techniques Insecticides - metabolism Insecticides - toxicity Male Medical sciences Microscopy, Electron Middle Aged Pesticides, fertilizers and other agrochemicals toxicology Propane - analogs & derivatives Propane - metabolism Propane - toxicity rat Rats Rats, Wistar testicular cells Testis - cytology Testis - drug effects Testis - metabolism Testis - radiation effects Testis - ultrastructure Toxicology x rays X-Rays - adverse effects 4-NQO rat DBCP DNA damage testicular cells x rays human Human Reproduction diseases Rat Toxicity Interspecific comparison Rodentia Pesticides Fumigant Germinal cell Metabolism Testicle In vitro Vertebrata Chromosome break Mammalia Animal DNA Male genital diseases
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Summary:	Preparations of testicular cells from human organ transplant donors and from Wistar rats were compared with respect to their composition of the different testicular cell types, their ability to metabolize 1,2-dibromo-3-chloropropane (DBCP), and their relative sensitivity to induction of DNA single strand breaks and alkali labile sites (ssDNA breaks) after treatment with DBCP, 4-nitroquinoline N-oxide (4-NQO), and x rays. Flow cytometric and microscopic analysis demonstrated that the interindividual variation in the composition of testicular cell types was considerably greater in the human tissue than in that from rats. The in vitro metabolic activation of DBCP (50 to 250 μM), measured as radiolabel covalently bound to macromolecules, was three-fold faster in rat testicular cells compared to human testicular cells. X rays (1 to 10 Gy) and 4-NQO (0.5 to 2.5 μM) induced ssDNA breaks to a similar extent in both human and rat testicular cells as measured by single cell gel electrophoresis (SCGE) and alkaline niter elution. In contrast, 1,2-dibromo-3-chloropropane (DBCP) (3 to 300 μM) caused no significant DNA damage in human testicular cells, whereas in rats there was a clear concentration-dependent increase in ssDNA breaks. The data show that, compared to rats, testicular cells from humans are less efficient in activating DBCP to metabolites binding covalently to macromolecules. However, from the rate of covalent binding observed one would expect a significant degree of DBCP-induced ssDNA breaks in the human testicular cells. The low level of DBCP-induced ssDNA breaks in human testicular cells could indicate that different reactive DBCP metabolites are involved in binding to cellular macromolecules compared to DNA damage, or that different rates of DNA repair exist in human and rat testicular cells.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0890-6238 1873-1708
DOI:	10.1016/0890-6238(95)02018-7