Aleutian mink disease parvovirus infection of K562 cells is antibody-dependent and is mediated via an Fc(gamma)RII receptor

Aleutian mink disease parvovirus infection of K562 cells is antibody-dependent and is mediated via an Fc(gamma)RII receptor

Aleutian mink disease parvovirus (ADV) infects macrophages but infection is restricted. Using the human macrophage cell line U937, we reported infection to be antibody-dependent. Recently, we learned that the U937 cells were contaminated with the human cell line, K562. To clarify which cell line act...

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Bibliographic Details
Published in:Archives of virology Vol. 142; no. 2; pp. 363 - 373
Main Authors: Dworak, L.J, Wolfinbarger, J.B, Bloom, M.E
Format: Journal Article
Language:English
Published: Wien Springer 1997
New York, NY Springer Nature B.V
Subjects:
Aleutian Mink Disease - immunology
Aleutian Mink Disease Virus
Animals
Antibodies - immunology
Biological and medical sciences
biological resistance
Cell Line
cell lines
Cells
Disease
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Viral - immunology
Humans
infection
Infections
Mice
Microbiology
Mink
Parvovirus
Receptors, Fc - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
Human
Parvovirus
Antibody
IgG
Host virus relation
In vitro
Virus
Cell line
Parvoviridae
Infectivity
Aleutian mink disease virus
Virus penetration
Parvovirinae
Macrophage
Biological receptor
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Summary:Aleutian mink disease parvovirus (ADV) infects macrophages but infection is restricted. Using the human macrophage cell line U937, we reported infection to be antibody-dependent. Recently, we learned that the U937 cells were contaminated with the human cell line, K562. To clarify which cell line actually supported ADV infection, we studied the antibody-dependent enhancement (ADE) of ADV infection in pure lines of U937 and K562. After infection with ADV complexed with mink anti-ADV antibody, up to 10% of the K562 cells, but none of the U937 cells showed evidence of infection. These results indicated that K562 cells, but not U937 cells, were susceptible to ADV. ADV-infected cultures of K562 were sorted by flow cytometry after staining with a murine monoclonal antibody (IV.3) directed against Fc(gamma)RII. Only cells that were IV.3 positive supported ADV gene expression. Also, preincubation of K562 cells with IV.3 blocked ADV infection by more than 90%. These results established that the ADE of ADV in K562 is Fc(gamma)RIIA-mediated.
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ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050082