Search Results - "Duyvesteyn, Helen M.E."

Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike by Huo, Jiandong, Zhao, Yuguang, Ren, Jingshan, Zhou, Daming, Duyvesteyn, Helen M.E., Ginn, Helen M., Carrique, Loic, Malinauskas, Tomas, Ruza, Reinis R., Shah, Pranav N.M., Tan, Tiong Kit, Rijal, Pramila, Coombes, Naomi, Bewley, Kevin R., Tree, Julia A., Radecke, Julika, Paterson, Neil G., Supasa, Piyada, Mongkolsapaya, Juthathip, Screaton, Gavin R., Carroll, Miles, Townsend, Alain, Fry, Elizabeth E., Owens, Raymond J., Stuart, David I.

“…There are as yet no licensed therapeutics for the COVID-19 pandemic. The causal coronavirus (SARS-CoV-2) binds host cells via a trimeric spike whose receptor…”
Get full text

Neutralization potency of monoclonal antibodies recognizing dominant and subdominant epitopes on SARS-CoV-2 Spike is impacted by the B.1.1.7 variant by Graham, Carl, Seow, Jeffrey, Huettner, Isabella, Khan, Hataf, Kouphou, Neophytos, Acors, Sam, Winstone, Helena, Pickering, Suzanne, Galao, Rui Pedro, Dupont, Liane, Lista, Maria Jose, Jimenez-Guardeño, Jose M., Laing, Adam G., Wu, Yin, Joseph, Magdalene, Muir, Luke, van Gils, Marit J., Ng, Weng M., Duyvesteyn, Helen M.E., Zhao, Yuguang, Bowden, Thomas A., Shankar-Hari, Manu, Rosa, Annachiara, Cherepanov, Peter, McCoy, Laura E., Hayday, Adrian C., Neil, Stuart J.D., Malim, Michael H., Doores, Katie J.

“…Interaction of the SARS-CoV-2 Spike receptor binding domain (RBD) with the receptor ACE2 on host cells is essential for viral entry. RBD is the dominant target…”
Get full text

The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants by Liu, Chang, Zhou, Daming, Nutalai, Rungtiwa, Duyvesteyn, Helen M.E., Tuekprakhon, Aekkachai, Ginn, Helen M., Dejnirattisai, Wanwisa, Supasa, Piyada, Mentzer, Alexander J., Wang, Beibei, Case, James Brett, Zhao, Yuguang, Skelly, Donal T., Chen, Rita E., Johnson, Sile Ann, Ritter, Thomas G., Mason, Chris, Malik, Tariq, Temperton, Nigel, Paterson, Neil G., Williams, Mark A., Hall, David R., Clare, Daniel K., Howe, Andrew, Goulder, Philip J.R., Fry, Elizabeth E., Diamond, Michael S., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the…”
Get full text

Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike by Huo, Jiandong, Zhao, Yuguang, Ren, Jingshan, Zhou, Daming, Duyvesteyn, Helen M.E., Ginn, Helen M., Carrique, Loic, Malinauskas, Tomas, Ruza, Reinis R., Shah, Pranav N.M., Tan, Tiong Kit, Rijal, Pramila, Coombes, Naomi, Bewley, Kevin R., Tree, Julia A., Radecke, Julika, Paterson, Neil G., Supasa, Piyada, Mongkolsapaya, Juthathip, Screaton, Gavin R., Carroll, Miles, Townsend, Alain, Fry, Elizabeth E., Owens, Raymond J., Stuart, David I.

Get full text

Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins by Ebrahim, Ali, Moreno-Chicano, Tadeo, Appleby, Martin V, Chaplin, Amanda K, Beale, John H, Sherrell, Darren A, Duyvesteyn, Helen M E, Owada, Shigeki, Tono, Kensuke, Sugimoto, Hiroshi, Strange, Richard W, Worrall, Jonathan A R, Axford, Danny, Owen, Robin L, Hough, Michael A

“…An approach is demonstrated to obtain, in a sample- and time-efficient manner, multiple dose-resolved crystal structures from room-temperature protein…”
Get full text

High-throughput structures of protein-ligand complexes at room temperature using serial femtosecond crystallography by Moreno-Chicano, Tadeo, Ebrahim, Ali, Axford, Danny, Appleby, Martin V, Beale, John H, Chaplin, Amanda K, Duyvesteyn, Helen M E, Ghiladi, Reza A, Owada, Shigeki, Sherrell, Darren A, Strange, Richard W, Sugimoto, Hiroshi, Tono, Kensuke, Worrall, Jonathan A R, Owen, Robin L, Hough, Michael A

“…High-throughput X-ray crystal structures of protein-ligand complexes are critical to pharmaceutical drug development. However, cryocooling of crystals and…”
Get full text

Towards in cellulo virus crystallography by Duyvesteyn, Helen M. E., Ginn, Helen M., Pietilä, Maija K., Wagner, Armin, Hattne, Johan, Grimes, Jonathan M., Hirvonen, Elina, Evans, Gwyndaf, Parsy, Marie-Laure, Sauter, Nicholas K., Brewster, Aaron S., Huiskonen, Juha T., Stuart, David I., Sutton, Geoff, Bamford, Dennis H.

“…Viruses are a significant threat to both human health and the economy, and there is an urgent need for novel anti-viral drugs and vaccines. High-resolution…”
Get full text

ChAdOx1 COVID vaccines express RBD open prefusion SARS-CoV-2 spikes on the cell surface by Ni, Tao, Mendonça, Luiza, Zhu, Yanan, Howe, Andrew, Radecke, Julika, Shah, Pranav M., Sheng, Yuewen, Krebs, Anna-Sophia, Duyvesteyn, Helen M.E., Allen, Elizabeth, Lambe, Teresa, Bisset, Cameron, Spencer, Alexandra, Morris, Susan, Stuart, David I., Gilbert, Sarah, Zhang, Peijun

“…Vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been proven to be an effective means of decreasing COVID-19 mortality,…”
Get full text

Antibody evasion by the P.1 strain of SARS-CoV-2 by Dejnirattisai, Wanwisa, Zhou, Daming, Supasa, Piyada, Liu, Chang, Mentzer, Alexander J., Ginn, Helen M., Zhao, Yuguang, Duyvesteyn, Helen M.E., Tuekprakhon, Aekkachai, Nutalai, Rungtiwa, Wang, Beibei, López-Camacho, César, Slon-Campos, Jose, Walter, Thomas S., Skelly, Donal, Costa Clemens, Sue Ann, Naveca, Felipe Gomes, Nascimento, Valdinete, Nascimento, Fernanda, Fernandes da Costa, Cristiano, Resende, Paola Cristina, Pauvolid-Correa, Alex, Siqueira, Marilda M., Dold, Christina, Levin, Robert, Dong, Tao, Pollard, Andrew J., Knight, Julian C., Crook, Derrick, Lambe, Teresa, Clutterbuck, Elizabeth, Bibi, Sagida, Flaxman, Amy, Bittaye, Mustapha, Belij-Rammerstorfer, Sandra, Gilbert, Sarah C., Carroll, Miles W., Klenerman, Paul, Barnes, Eleanor, Dunachie, Susanna J., Paterson, Neil G., Williams, Mark A., Hall, David R., Hulswit, Ruben J.G., Bowden, Thomas A., Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…Terminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses to the virus spike derived from…”
Get full text

Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 by Huo, Jiandong, Le Bas, Audrey, Ruza, Reinis R., Duyvesteyn, Helen M. E., Mikolajek, Halina, Malinauskas, Tomas, Tan, Tiong Kit, Rijal, Pramila, Dumoux, Maud, Ward, Philip N., Ren, Jingshan, Zhou, Daming, Harrison, Peter J., Weckener, Miriam, Clare, Daniel K., Vogirala, Vinod K., Radecke, Julika, Moynié, Lucile, Zhao, Yuguang, Gilbert-Jaramillo, Javier, Knight, Michael L., Tree, Julia A., Buttigieg, Karen R., Coombes, Naomi, Elmore, Michael J., Carroll, Miles W., Carrique, Loic, Shah, Pranav N. M., James, William, Townsend, Alain R., Stuart, David I., Owens, Raymond J., Naismith, James H.

“…The SARS-CoV-2 virus is more transmissible than previous coronaviruses and causes a more serious illness than influenza. The SARS-CoV-2 receptor binding domain…”
Get full text

Potent cross-reactive antibodies following Omicron breakthrough in vaccinees by Nutalai, Rungtiwa, Zhou, Daming, Tuekprakhon, Aekkachai, Ginn, Helen M., Supasa, Piyada, Liu, Chang, Huo, Jiandong, Mentzer, Alexander J., Duyvesteyn, Helen M.E., Dijokaite-Guraliuc, Aiste, Skelly, Donal, Ritter, Thomas G., Amini, Ali, Bibi, Sagida, Adele, Sandra, Johnson, Sile Ann, Constantinides, Bede, Webster, Hermione, Temperton, Nigel, Klenerman, Paul, Barnes, Eleanor, Dunachie, Susanna J., Crook, Derrick, Pollard, Andrew J., Lambe, Teresa, Goulder, Philip, Paterson, Neil G., Williams, Mark A., Hall, David R., Mongkolsapaya, Juthathip, Fry, Elizabeth E., Dejnirattisai, Wanwisa, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…Highly transmissible Omicron variants of SARS-CoV-2 currently dominate globally. Here, we compare neutralization of Omicron BA.1, BA.1.1, and BA.2. BA.2 RBD…”
Get full text

Site-Specific Steric Control of SARS-CoV‑2 Spike Glycosylation by Allen, Joel D, Chawla, Himanshi, Samsudin, Firdaus, Zuzic, Lorena, Shivgan, Aishwary Tukaram, Watanabe, Yasunori, He, Wan-ting, Callaghan, Sean, Song, Ge, Yong, Peter, Brouwer, Philip J. M, Song, Yutong, Cai, Yongfei, Duyvesteyn, Helen M. E, Malinauskas, Tomas, Kint, Joeri, Pino, Paco, Wurm, Maria J, Frank, Martin, Chen, Bing, Stuart, David I, Sanders, Rogier W, Andrabi, Raiees, Burton, Dennis R, Li, Sai, Bond, Peter J, Crispin, Max

“…A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity. The abundance of N-linked glycans…”
Get full text

Machining protein microcrystals for structure determination by electron diffraction by Duyvesteyn, Helen M. E., Kotecha, Abhay, Ginn, Helen M., Hecksel, Corey W., Beale, Emma V., de Haas, Felix, Evans, Gwyndaf, Zhang, Peijun, Chiu, Wah, Stuart, David I.

“…We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This…”
Get full text

Structural and mechanistic characterization of bifunctional heparan sulfate N-deacetylase-N-sulfotransferase 1 by Mycroft-West, Courtney J., Abdelkarim, Sahar, Duyvesteyn, Helen M. E., Gandhi, Neha S., Skidmore, Mark A., Owens, Raymond J., Wu, Liang

“…Heparan sulfate (HS) polysaccharides are major constituents of the extracellular matrix, which are involved in myriad structural and signaling processes…”
Get full text

Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike by Duyvesteyn, Helen M E, Dijokaite-Guraliuc, Aiste, Liu, Chang, Supasa, Piyada, Kronsteiner, Barbara, Jeffery, Katie, Stafford, Lizzie, Klenerman, Paul, Dunachie, Susanna J, Mongkolsapaya, Juthathip, Fry, Elizabeth E, Ren, Jingshan, Stuart, David I, Screaton, Gavin R

“…BA.2.87.1 represents a major shift in the BA.2 lineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is unusual in having two lengthy…”
Get more information

A delicate balance between antibody evasion and ACE2 affinity for Omicron BA.2.75 by Huo, Jiandong, Dijokaite-Guraliuc, Aiste, Liu, Chang, Zhou, Daming, Ginn, Helen M., Das, Raksha, Supasa, Piyada, Selvaraj, Muneeswaran, Nutalai, Rungtiwa, Tuekprakhon, Aekkachai, Duyvesteyn, Helen M.E., Mentzer, Alexander J., Skelly, Donal, Ritter, Thomas G., Amini, Ali, Bibi, Sagida, Adele, Sandra, Johnson, Sile Ann, Paterson, Neil G., Williams, Mark A., Hall, David R., Plowright, Megan, Newman, Thomas A.H., Hornsby, Hailey, de Silva, Thushan I., Temperton, Nigel, Klenerman, Paul, Barnes, Eleanor, Dunachie, Susanna J., Pollard, Andrew J., Lambe, Teresa, Goulder, Philip, Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have caused successive global waves of infection. These variants, with multiple…”
Get full text

A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids by Clarke, John D, Duyvesteyn, Helen M E, Perez-Martin, Eva, Latišenko, Undīne, Porta, Claudine, Humphreys, Kathleen V, Hay, Abigail L, Ren, Jingshan, Fry, Elizabeth E, van den Born, Erwin, Charleston, Bryan, Bonnet-Di Placido, Marie, Owens, Raymond J, Stuart, David I, Hammond, John A

“…Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct…”
Get more information

Signature of Antibody Domain Exchange by Native Mass Spectrometry and Collision-Induced Unfolding by Watanabe, Yasunori, Vasiljevic, Snezana, Allen, Joel D, Seabright, Gemma E, Duyvesteyn, Helen M. E, Doores, Katie J, Crispin, Max, Struwe, Weston B

“…The development of domain-exchanged antibodies offers a route to high-affinity targeting to clustered multivalent epitopes, such as those associated with viral…”
Get full text

The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86 by Zhou, Daming, Supasa, Piyada, Liu, Chang, Dijokaite-Guraliuc, Aiste, Duyvesteyn, Helen M. E., Selvaraj, Muneeswaran, Mentzer, Alexander J., Das, Raksha, Dejnirattisai, Wanwisa, Temperton, Nigel, Klenerman, Paul, Dunachie, Susanna J., Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…Under pressure from neutralising antibodies induced by vaccination or infection the SARS-CoV-2 spike gene has become a hotspot for evolutionary change, leading…”
Get full text

Emerging variants develop total escape from potent monoclonal antibodies induced by BA.4/5 infection by Liu, Chang, Das, Raksha, Dijokaite-Guraliuc, Aiste, Zhou, Daming, Mentzer, Alexander J., Supasa, Piyada, Selvaraj, Muneeswaran, Duyvesteyn, Helen M. E., Ritter, Thomas G., Temperton, Nigel, Klenerman, Paul, Dunachie, Susanna J., Paterson, Neil G., Williams, Mark A., Hall, David R., Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.

“…The rapid evolution of SARS-CoV-2 is driven in part by a need to evade the antibody response in the face of high levels of immunity. Here, we isolate spike (S)…”
Get full text