Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to...

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Bibliographic Details
Published in:	Narra J Vol. 4; no. 2; p. e774
Main Authors:	Jamaluddin, Indra P, Musa, Susan H, Ethica, Stalis N, Ansori, Arif Nm, Yosephi, Valensa, Atmaja, Peter Y, Murtadlo, Ahmad Aa, Sahadewa, Sukma, Durry, Fara D, Rebezov, Maksim, Derkho, Marina, Naw, Sin W, Zainul, Rahadian, Rachmawati, Kadek
Format:	Journal Article
Language:	English
Published:	Indonesia 01-08-2024
Subjects:	Child, Preschool DNA Gyrase - genetics Humans Indonesia Male Polymerase Chain Reaction - methods Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - diagnosis Pseudomonas Infections - microbiology RNA, Ribosomal, 16S - genetics Suppuration - microbiology Wound Infection - diagnosis Wound Infection - microbiology Indonesia 16S rRNA Pseudomonas aeruginosa gyrB PCR sequencing
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Summary:	Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and genes in using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gene. The BLAST program demonstrated that the sample had 99.89% similarity with strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gene exhibited 99.10% similarity with the strain PSA-1.2 (accession code KP172300.1).
ISSN:	2807-2618 2807-2618
DOI:	10.52225/narra.v4i2.774