High Genetic Stability of the Region Coding for the Structural Proteins of Yellow Fever Virus Strain 17D

Unité de Virologie Moléculaire, UA CNRS 545, Institut Pasteur, 75724 Paris Cedex 15, France and 1 Institut Pasteur, B.P. 220, Dakar, Sénégal The genome of the Pasteur 17D-204 vaccine strain of yellow fever virus has been cloned into pBR327. The inserts of recombinant plasmids were analysed by restri...

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Bibliographic Details
Published in:	Journal of general virology Vol. 68; no. 8; pp. 2245 - 2247
Main Authors:	Despres, Philippe, Cahour, Annie, Dupuy, Alfred, Deubel, Vincent, Bouloy, Michele, Digoutte, Jean Pierre, Girard, Marc
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-08-1987 Society for General Microbiology
Subjects:	Arboviroses Biological and medical sciences Cloning, Molecular DNA - analysis DNA Restriction Enzymes Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Viral Genetics Human viral diseases Infectious diseases Medical sciences Microbiology Nucleotide Mapping Plasmids Tropical medicine Tropical viral diseases Viral diseases Viral Proteins - genetics Virology Yellow fever yellow fever virus Yellow fever virus - genetics 5' terminal-Sequence Genetic variability RNA Vaccine strain Togaviridae Structure gene Flavivirus Virus Proteins Restriction map Arbovirus Molecular cloning Yellow fever virus
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Summary:	Unité de Virologie Moléculaire, UA CNRS 545, Institut Pasteur, 75724 Paris Cedex 15, France and 1 Institut Pasteur, B.P. 220, Dakar, Sénégal The genome of the Pasteur 17D-204 vaccine strain of yellow fever virus has been cloned into pBR327. The inserts of recombinant plasmids were analysed by restriction cleavage pattern and compared with that of the genome of another substrain previously cloned and sequenced. Ten of the overlapping inserts were found to contain the sequence of the complete genome. We have sequenced approximately 3680 bases of the 5' region which codes for the C, M and E structural proteins and the NS1 non-structural protein. This sequence is the same as that reported previously, indicating a remarkable stability of these two vaccine substrains. Keywords: YFV, genetic stability, structural proteins Present address: Centers for Disease Control, Fort Collins, Colorado 80522, U.S.A. Received 3 March 1987; accepted 28 April 1987.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-68-8-2245