Search Results - "Dunlap, R.Bruce"

Correction for light absorption in fluorescence studies of protein-ligand interactions by Birdsall, B, King, R W, Wheeler, M R, Lewis, Jr, C A, Goode, S R, Dunlap, R B, Roberts, G C

“…It is shown that absorption of the excitation light can lead to substantial systematic errors in fluorescence measurements of equilibrium constants for…”
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Reaction of alcohols (via the Mitsunobu reaction) and alkyl halides with chiral selone derivatizing agents by Wu, Ruilian, Odom, Jerome D, Dunlap, R.Bruce, Silks, Louis A

“…Coupling of a selone chiral derivatizing agent (CDA) to d, l-alkyl halides gives Se-alkylated adducts in yields ranging from 76–97%. Reaction of d, l-alcohols…”
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Kinetic Studies on Drug-Resistant Variants of Escherichia coli Thymidylate Synthase: Functional Effects of Amino Acid Substitutions at Residue 4 by Mahdavian, Elahe, Spencer, H.Trent, Dunlap, R.Bruce

“…A naturally occurring mutant of human thymidylate synthase (hTS) that contains a Tyr to His mutation at residue 33 was found to confer 4-fold resistance to…”
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Differences in natural ligand and fluoropyrimidine binding to human thymidylate synthase identified by transient-state spectroscopic and continuous variation methods by Felder, Takita, Dunlap, R.Bruce, Dix, Daniel, Spencer, Trent

“…Thymidylate synthase (TS) is a central target for the design of chemotherapeutic agents due to its vital role in DNA synthesis. Structural studies of binary…”
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High expression and steady-state kinetic characterization of methionine site-directed mutants of Escherichia coli methionyl- and selenomethionyl-dihydrofolate reductase by Shaw, David, Odom, JeromeD, Dunlap, R.Bruce

“…A high expression system that produces Escherichia coli dihydrofolate reductase (DHFR) at 30% total cellular protein was constructed. This expression vector,…”
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Development of a trichloroacetic acid precipitation assay for covalent adducts of thymidylate synthase by Cisneros, R J, Dunlap, R B

“…The use of trichloroacetic acid as a protein precipitant and denaturant in the quantitative measurement of covalent complexes of thymidylate synthase is…”
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Site-directed mutagenesis of mouse thymidylate synthase: alteration of Arg44 to Val44 in a conserved loop guarding the active site has striking effects on catalysis and nucleotide binding by Zhang, H C, Cisneros, R J, Deng, W L, Johnson, L F, Dunlap, R B

“…The arginine located at position 44 of mouse thymidylate synthase is in a highly conserved loop that is in close proximity to the active site cleft of the…”
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Efficient synthesis of mouse thymidylate synthase in Escherichia coli by Zhang, H C, Cisneros, R J, Dunlap, R B, Johnson, L F

“…The coding region of the mouse thymidylate synthase (TS)-encoding cDNA (ts) was inserted downstream from the phage T7 promoter and translation initiation…”
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Characterization of the covalent chromatography of thymidylate synthase on thiopropyl-Sepharose 6B by Bradshaw, T P, Dunlap, R B

“…We have examined the covalent chromatography of Lactobacillus casei thymidylate synthase on thiopropyl-Sepharose 6B resin. This enzyme is a dimer of identical…”
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Isolation of the covalent binary complex of 5-fluorodeoxyuridylate and thymidylate synthetase by trichloroacetic acid precipitation by Moore, M A, Ahmed, F, Dunlap, R B

“…Strong chemical evidence for the existence of a covalent binary complex between 5-fluorodeoxyuridylate and thymidylate synthetase was provided by the isolation…”
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Characterization of a new sulfhydryl group reagent: 6, 6'-diselenobis-(3-nitrobenzoic acid), a selenium analog of Ellman's reagent by Luthra, N P, Dunlap, R B, Odom, J D

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Characterization of the parameters affecting covalent binding stoichiometry in binary and ternary complexes of thymidylate synthase by Cisneros, R J, Dunlap, R B

“…Covalent binding stoichiometries for both the enzyme:5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP) binary complex and the…”
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Crystallization and crystallographic data for new forms of thymidylate synthase from Lactobacillus casei by Tykarska, E, Lebioda, L, Bradshaw, T P, Dunlap, R B

“…Several new crystal forms of thymidylate synthase (5,10-methlenetetrahydrofolate:dUMP C-methyltransferase; EC 2.1.1.45) were obtained by controlled pH change…”
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A nitrocellulose-filter assay for the binary complex of 5-fluorodeoxyuridylate and Lactobacillus casei thymidylate synthetase by Ahmed, F, Moore, M A, Dunlap, R B

“…The interaction of 5-fluorodeoxyuridylate (FdUMP) with thymidylate synthetase to form a binary complex has been widely reported, yet previous attempts to…”
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Interactions between tetrahydrofolate and palladium(II) complexes by Donato, H, Dunlap, R B

“…Interactions between folate derivatives and palladium(II) complexes are monitored in 1 : 1 molar ratio mixtures by circular dichroic spectra. The results are…”
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Chemical modification of Lactobacillus casei thymidylate synthetase with 1-fluoro-2,4-dinitrobenzene and 1,5-difluoro-2,4-dinitrobenzene by Munroe, W A, Dunlap, R B

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Modification of tyrosine residues in dihydrofolate reductase from Lactobacillus casei by Rosson, D, Dunlap, R B

“…Dihydrofolate reductase from Lactobacillus casei was inactivated by reaction with tetranitromethane and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. Loss of…”
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Carbodiimide-dependent inactivation of dihydrofolate reductase by Reinsch, J W, Dunlap, R B

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Essential arginyl residues in thymidylate synthetase by Cipollo, K L, Dunlap, R B

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Purification and characterization of recombinant mouse thymidylate synthase by Zhang, H C, Cisneros, R J, Deng, W L, Zapf, J W, Johnson, L F, Dunlap, R B

“…Recombinant mouse thymidylate synthase (TS) expressed at high levels in Escherichia coli was purified to homogeneity in greater than 70% yield by a rapid…”
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