Freeze artifact on full-field optical coherence tomography skin imaging

Full-field optical coherence tomography (FFOCT) is an emerging imaging technique for rapid histological analysis. As FFOCT is introduced into the Mohs workflow, it is important to document the effect of tissue freezing on FFOCT images and any effect FFOCT has on frozen sections. Our study aimed to e...

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Bibliographic Details
Published in:	Skin research and technology Vol. 24; no. 1; p. 54
Main Authors:	Durkin, J R, Ogrich, L, Morales-Neira, D, Dundumalla, S, Abdelmalek, M
Format:	Journal Article
Language:	English
Published:	England 01-02-2018
Subjects:	Adipocytes - pathology Collagen - analysis Cryopreservation Eccrine Glands - diagnostic imaging Eccrine Glands - pathology Epidermis - diagnostic imaging Epidermis - pathology Humans Mohs Surgery Preoperative Care - methods Sebaceous Glands - diagnostic imaging Sebaceous Glands - pathology Skin - diagnostic imaging Skin - pathology Tomography, Optical Coherence - methods Mohs skin imaging FFOCT full field optical coherence tomography freeze artifact
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Summary:	Full-field optical coherence tomography (FFOCT) is an emerging imaging technique for rapid histological analysis. As FFOCT is introduced into the Mohs workflow, it is important to document the effect of tissue freezing on FFOCT images and any effect FFOCT has on frozen sections. Our study aimed to evaluate the changes on FFOCT images after tissue freezing as well as FFOCT imaging effects on frozen sectioning. Six normal skin specimens were imaged using FFOCT and subsequently frozen using a cryostat. The specimens were then reimaged using FFOCT and compared for any differences. To evaluate the effect of FFOCT imaging on frozen sections, five normal skin specimens were bisected and one half was imaged using FFOCT. Both halves underwent frozen sectioning and analysis. Significant changes in the fat, sebaceous glands, eccrine glands, and dermal collage and minimal changes in the epidermis were seen after freezing. No effect on frozen sectioning was seen after FFOCT imaging. As FFOCT is studied for use in Mohs, clinicians should be aware that freezing prior to imaging introduces significant artifact in the FFOCT image. If possible, specimens should undergo imaging prior to being frozen.
ISSN:	1600-0846
DOI:	10.1111/srt.12389