GADD45α and γ interaction with CDK11p58 regulates SPDEF protein stability and SPDEF-mediated effects on cancer cell migration

The epithelium-specific Ets transcription factor, SPDEF, plays a critical role in metastasis of prostate and breast cancer cells. While enhanced SPDEF expression blocks migration and invasion, knockdown of SPDEF expression enhances migration, invasion, and metastasis of cancer cells. SPDEF expressio...

Full description

Saved in:

Bibliographic Details
Published in:	Oncotarget Vol. 7; no. 12; pp. 13865 - 13879
Main Authors:	Tamura, Rodrigo E, Paccez, Juliano D, Duncan, Kristal C, Morale, Mirian G, Simabuco, Fernando M, Dillon, Simon, Correa, Ricardo G, Gu, Xuesong, Libermann, Towia A, Zerbini, Luiz F
Format:	Journal Article
Language:	English
Published:	United States Impact Journals LLC 22-03-2016
Subjects:	Apoptosis Cell Cycle Proteins - metabolism Cell Movement Cell Proliferation Cyclin D3 - metabolism Gene Expression Regulation, Neoplastic Humans Intracellular Signaling Peptides and Proteins - metabolism Male Nuclear Proteins - metabolism Phosphorylation Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Protein Interaction Maps Protein Stability Proteolysis Proto-Oncogene Proteins c-ets - chemistry Proto-Oncogene Proteins c-ets - metabolism Research Paper Tumor Cells, Cultured GADD45 migration invasion CDK11p58 SPDEF
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The epithelium-specific Ets transcription factor, SPDEF, plays a critical role in metastasis of prostate and breast cancer cells. While enhanced SPDEF expression blocks migration and invasion, knockdown of SPDEF expression enhances migration, invasion, and metastasis of cancer cells. SPDEF expression and activation is tightly regulated in cancer cells; however, the precise mechanism of SPDEF regulation has not been explored in detail. In this study we provide evidence that the cell cycle kinase CDK11p58, a protein involved in G2/M transition and degradation of several transcription factors, directly interacts with and phosphorylates SPDEF on serine residues, leading to subsequent ubiquitination and degradation of SPDEF through the proteasome pathway. As a consequence of CDK11p58 mediated degradation of SPDEF, this loss of SPDEF protein results in increased prostate cancer cell migration and invasion. In contrast, knockdown of CDK11p58 protein expression by interfering RNA or SPDEF overexpression inhibit migration and invasion of cancer cells. We demonstrate that CDK11p58 mediated degradation of SPDEF is attenuated by Growth Arrest and DNA damage-inducible 45 (GADD45) α and , two proteins inducing G2/M cell cycle arrest. We show that GADD45 α and γ, directly interact with CDK11p58 and thereby inhibit CDK11p58 activity, and consequentially SPDEF phosphorylation and degradation, ultimately reducing prostate cancer cell migration and invasion. Our findings provide new mechanistic insights into the complex regulation of SPDEF activity linked to cancer metastasis and characterize a previously unidentified SPDEF/CDK11p58/GADD45α/γ pathway that controls SPDEF protein stability and SPDEF-mediated effects on cancer cell migration and invasion.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally and should be considered senior authors
ISSN:	1949-2553 1949-2553
DOI:	10.18632/oncotarget.7355