Search Results - "Duhé, R J"

Characterization of Active and Inactive Forms of the JAK2 Protein-tyrosine Kinase Produced via the Baculovirus Expression Vector System by Duhé, R J, Farrar, W L

“…Three forms of rat JAK2 (type 2 Janus tyrosine kinase) were produced via the baculovirus expression vector system. Recombinant baculoviruses encoded either the…”
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Structural and mechanistic aspects of Janus kinases: how the two-faced god wields a double-edged sword by Duhé, R J, Farrar, W L

“…The Janus family of protein-tyrosine kinases has long been known to function in signal transduction pathways initiated by a host of cytokines. A brief overview…”
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Cloning of the gene encoding rat JAK2, a protein tyrosine kinase by Duhé, Roy J., Rui, Hallgeir, Greenwood, John D., Garvey, Kevin, Farrar, William L.

“…A complete cDNA clone encoding the rat JAK2 protein tyrosine kinase was isolated from an Nb2-SP (rat pre-T lymphoma cell line) cDNA library. The nucleotide…”
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Molecular cloning of FKHRL1P2, a member of the developmentally regulated fork head domain transcription factor family by DaSilva, Luis, Kirken, Robert A., Taub, Dennis D., Evans, Gerald A., Duhé, Roy J., Bailey, Melissa A., Farrar, William L.

“…Here we report the expression of a fork head domain protein in human T helper cells. We cloned and characterized a fork head cDNA from human T helper cell mRNA…”
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Nitric Oxide and Thiol Redox Regulation of Janus Kinase Activity by Duhe, Roy J., Evans, Gerald A., Erwin, Rebecca A., Kirken, Robert A., Cox, George W., Farrar, William L.

“…The activation of Janus kinases (JAKs) is crucial for propagation of the proliferative response initiated by many cytokines. The proliferation of various cell…”
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Mechanisms of cytokine signal transduction: IL-2, IL-4 and prolactin as hematopoietin receptor models by Kirken, Robert A, Evans, Gerald A, Duhé, Roy J, DaSilva, Luis, Malabarba, M.Grazia, Erwin, Rebecca A, Farrar, William L

“…Cytokines, hormones and hematopoietic growth factors transduce biological signals across the cell membrane via a highly conserved family of single…”
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Oxidation of critical cysteine residues of type I adenylyl cyclase by o-iodosobenzoate or nitric oxide reversibly inhibits stimulation by calcium and calmodulin by DUHE, R. J, NIELSEN, M. D, DITTMAN, A. H, VILLACRES, E. C, EUI-JU CHOI, STORM, D. R

“…The calmodulin binding domain of the type I adenylyl cyclase has recently been identified as an amino acid sequence (residues 495-522) that contains 2 cysteine…”
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Characterization of the in vitro kinase activity of a partially purified soluble GST/JAK2 fusion protein by Duhé, Roy J, Clark, Emily A, Farrar, William L

“…The biochemical and biophysical characteristics of Janus protein-tyrosine kinases (JAKs), which are essential early mediators of cytokine-initiated signal…”
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Defining Functional Domains of Ku80: DNA End Binding and Survival after Radiation by Osipovich, O., Duhe, R.J., Hasty, P., Durum, S.K., Muegge, K.

“…The Ku heterodimeric protein (Ku80/Ku70) is an essential component of the double-strand break DNA repair pathway in mammalian cells. We have recently defined a…”
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Negative regulation of Janus kinases by Duhé, R J, Wang, L H, Farrar, W L

“…The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many…”
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L-1-N-methyl-4-mercaptohistidine disulfide, a potential endogenous regulator in the redox control of chloroplast coupling factor 1 in Dunaliella by Selman-Reimer, S, Duhe, R J, Stockman, B J, Selman, B R

“…A water-soluble, highly polar, heat-stable, small molecule has been isolated from cell-free extracts of the halotolerant green alga Dunaliella salina. This…”
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Janus Kinase 2 Activation by the Platelet-Activating Factor Receptor (PAFR): Roles of Tyk2 and PAFR C Terminus by Lukashova, Viktoria, Chen, Zhangguo, Duhe, Roy J, Rola-Pleszczynski, Marek, Stankova, Jana

“…Platelet-activating factor (PAF) is a phospholipid with multiple physiological and pathological actions. The PAF receptor (PAFR) belongs to the G…”
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N-Ethylmaleimide inhibition of the catalytic activities of the Dunaliella salina coupling factor 1 (CF1) and the restoration of the inhibition of the CF1 ATPase activity by N-ethylmaleimide by Selman-Reimer, S, Duhe, R J, Selman, B R

“…The sensitivity of the catalytic activities of the D. salina chloroplast coupling factor 1 (CF1) to chemical modification by N-ethylmaleimide has been…”
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Microinjected cDNA encoding JAK2 protein-tyrosine kinase induces DNA synthesis in NIH 3T3 cells by Smith, Mark R., Duhé, Roy J., Liu, Ya-lun, Farrar, William L.

“…Microinjection of expression plasmids encoding either JAK2 or hyperactive (NΔ661)rJAK2 into serum-starved NIH 3T3 cells resulted in 20–30-fold induction of DNA…”
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Emerging roles of targeted small molecule protein-tyrosine kinase inhibitors in cancer therapy by Smith, John K, Mamoon, Naila M, Duhé, Roy J

“…Targeted protein-tyrosine kinase inhibitors (PTKIs) comprise a new, rapidly evolving class of low molecular weight anticancer drugs. Two members of this class,…”
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Tyrosine Phosphorylation of the Janus Kinase 2 Activation Loop Is Essential for a High-Activity Catalytic State but Dispensable for a Basal Catalytic State by Chatti, Kiranam, Farrar, William L, Duhé, Roy J

“…The phosphorylation of an “activation loop” within protein kinases is commonly associated with establishing catalytic competence, and phosphorylation of the…”
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The dithiothreitol-stimulated dissociation of the chloroplast coupling factor 1 epsilon-subunit is reversible by Duhe, R J, Selman, B R

“…The chloroplast coupling factor 1 complex (CF1) contains an epsilon-subunit which inhibits the CF1 ATPase activity. Chloroform treatment of Chlamydomonas…”
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