Identification of host factors potentially involved in RTM-mediated resistance during potyvirus long distance movement

The long distance movement of potyviruses is a poorly understood step of the viral cycle. Only factors inhibiting this process, referred to as “Restricted TEV Movement” (RTM), have been identified in Arabidopsis thaliana . On the virus side, the potyvirus coat protein (CP) displays determinants requ...

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Bibliographic Details
Published in:	Archives of virology Vol. 162; no. 7; pp. 1855 - 1865
Main Authors:	Sofer, Luc, Cabanillas, Daniel Garcia, Gayral, Mathieu, Téplier, Rachèle, Pouzoulet, Jérôme, Ducousso, Marie, Dufin, Laurène, Bréhélin, Claire, Ziegler-Graff, Véronique, Brault, Véronique, Revers, Frédéric
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-07-2017 Springer Nature B.V Springer Verlag
Subjects:	Arabidopsis Arabidopsis - virology Biomedical and Life Sciences Biomedicine Capsid Proteins - physiology Chloroplasts Coat protein Gene Expression Regulation, Plant - physiology Gene Expression Regulation, Viral - physiology Infectious Diseases Life Sciences Localization Medical Microbiology Microscopy, Confocal Nicotiana - physiology Nicotiana - virology Original Article Phloem - metabolism Phloem - virology Plant Diseases - virology Plant Epidermis - cytology Plant Proteins - genetics Plant Proteins - metabolism Plastids Potyvirus - physiology Protein Transport Proteins Two-Hybrid System Techniques Vegetal Biology Virions Virology Coat Protein Y2HGold Yeast Strain Lettuce Mosaic Virus Band Emission Pass Filter Phloem Tissue
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Summary:	The long distance movement of potyviruses is a poorly understood step of the viral cycle. Only factors inhibiting this process, referred to as “Restricted TEV Movement” (RTM), have been identified in Arabidopsis thaliana . On the virus side, the potyvirus coat protein (CP) displays determinants required for long-distance movement and for RTM-based resistance breaking. However, the potyvirus CP was previously shown not to interact with the RTM proteins. We undertook the identification of Arabidopsis factors which directly interact with either the RTM proteins or the CP of lettuce mosaic virus (LMV). An Arabidopsis cDNA library generated from companion cells was screened with LMV CP and RTM proteins using the yeast two-hybrid system. Fourteen interacting proteins were identified. Two of them were shown to interact with CP and the RTM proteins suggesting that a multiprotein complex could be formed between the RTM proteins and virions or viral ribonucleoprotein complexes. Co-localization experiments in Nicotiana benthamiana showed that most of the viral and cellular protein pairs co-localized at the periphery of chloroplasts which suggests a putative role for plastids in this process.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-017-3292-6