Search Results - "Duewel, H S"

Structures of Aquifex aeolicus KDO8P Synthase in Complex with R5P and PEP, and with a Bisubstrate Inhibitor:  Role of Active Site Water in Catalysis by Wang, Jian, Duewel, Henry S, Woodard, Ronald W, Gatti, Domenico L

“…We have determined the crystal structures of the metalloenzyme 3-deoxy-d-manno-octulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with…”
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Elucidation of Solvent Exposure, Side-Chain Reactivity, and Steric Demands of the Trifluoromethionine Residue in a Recombinant Protein by Duewel, Henry S, Daub, Elisabeth, Robinson, Valerie, Honek, John F

“…When incorporated into proteins, fluorinated amino acids have been utilized as 19F NMR probes of protein structure and protein−ligand interactions, and as…”
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A metal bridge between two enzyme families. 3-deoxy-D-manno-octulosonate-8-phosphate synthase from Aquifex aeolicus requires a divalent metal for activity by Duewel, H S, Woodard, R W

“…The enzymes 3-deoxy-d-manno-octulosonic acid-8-phosphate synthase (KDO8PS) and 3-deoxy-d-arabino-heptulosonic acid-7-phosphate synthase (DAHPS) catalyze…”
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Crystal Structure of the Lytic Transglycosylase from Bacteriophage Lambda in Complex with Hexa-N-acetylchitohexaose by Leung, Adelaine K.-W, Duewel, Henry S, Honek, John F, Berghuis, Albert M

“…The three-dimensional structure of the lytic transglycosylase from bacteriophage lambda, also known as bacteriophage lambda lysozyme, complexed to the…”
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Functional and Biochemical Characterization of a Recombinant 3-Deoxy-d-manno-octulosonic Acid 8-Phosphate Synthase from the Hyperthermophilic Bacterium Aquifex aeolicus by Duewel, Henry S., Sheflyan, Galina Ya, Woodard, Ronald W.

“…The kdsA gene from the hyperthermophilic bacterium Aquifex aeolicus was cloned into a vector for expression in Escherichia coli and the kdsA gene product,…”
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Histidine 268 in 3-Deoxy-d-arabino-heptulosonic Acid 7-Phosphate Synthase Plays the Same Role as Histidine 202 in 3-Deoxy-d-manno-octulosonic Acid 8-Phosphate Synthase by Howe, David L., Duewel, Henry S., Woodard, Ronald W.

“…The enzyme 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAH 7-P) synthase (Phe) is inactivated by diethyl pyrocarbonate (DEPC). The inactivation is first order…”
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Identification of Essential Histidine Residues in 3-Deoxy-d-manno-octulosonic Acid 8-Phosphate Synthase:  Analysis by Chemical Modification with Diethyl Pyrocarbonate and Site-Directed Mutagenesis by Sheflyan, Galina Ya, Duewel, Henry S, Chen, Grace, Woodard, Ronald W

“…The enzyme 3-deoxy-d-manno-octulosonic acid 8-phosphate (KDO 8-P) synthase from Escherichia coli that catalyzes the aldol-type condensation of d-arabinose…”
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Substrate and metal complexes of 3-deoxy-D-manno-octulosonate-8-phosphate synthase from Aquifex aeolicus at 1.9-A resolution. Implications for the condensation mechanism by Duewel, H S, Radaev, S, Wang, J, Woodard, R W, Gatti, D L

“…3-Deoxy-D-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs from its Escherichia coli counterpart…”
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Investigations of the interactions of saccharides with the lysozyme from bacteriophage lambda by Duewel, Henry S., Daub, Elisabeth, Honek, John F.

“…The bacteriophage lambda R gene has been isolated into an Escherichia coli expression system and the R gene product, a lysozyme, has been overexpressed and…”
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Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation by Tanis, Keith Q., Veach, Darren, Duewel, Henry S., Bornmann, William G., Koleske, Anthony J.

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Functional and Biochemical Characterization of a Recombinant 3-Deoxy-manno-octulosonic Acid 8-Phosphate Synthase from the Hyperthermophilic Bacterium Aquifex aeolicus by Duewel, H S, Sheflyan, G Y, Woodard, R W

“…The kdsA gene from the hyperthermophilic bacterium Aquifex aeolicus was cloned into a vector for expression in Escherichia coli and the kdsA gene product,…”
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Substrate and Metal Complexes of 3-Deoxy-D-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Aa Resolution. Implications for the Condensation Mechanism by Duewel, H S, Radaev, S, Wang, J, Woodard, R W, Gatti, D L

“…3-Deoxy-D-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs from its Escherichia coli counterpart…”
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On-line strong cation exchange μ-HPLC-ESI-MS/MS for protein identification and process optimization by Le Bihan, Thierry, Duewel, Henry S, Figeys, Daniel

“…We have developed an on-line strong cation exchange (SCX)-ESI-MS/MS platform for the rapid identification of proteins contained in mixtures. This platform…”
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CNBr/formic acid reactions of methionine- and trifluoromethionine-containing lambda lysozyme: probing chemical and positional reactivity and formylation side reactions by mass spectrometry by Duewel, H S, Honek, J F

“…The cyanogen bromide (CNBr)/formic acid cleavage reactions of wild-type and trifluoromethionine (TFM)-containing recombinant lambda lysozyme were studied…”
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Function of His185 in Aquifex aeolicus 3-Deoxy- d- manno-octulosonate 8-Phosphate Synthase by Wang, Jian, Duewel, Henry S, Stuckey, Jeanne A, Woodard, Ronald W, Gatti, Domenico L

“…Aquifex aeolicus 3-deoxy- d- manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate…”
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