Water Sampling Module for Collecting and Concentrating Legionella pneumophila from Low-to-Medium Contaminated Environment

The detection of water contamination with is of critical importance to manufacturers of water processing equipment and public health entities dealing with water networks and distribution systems. Detection methods based on polymerase chain reaction or biosensor technologies require preconcentration...

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Bibliographic Details
Published in:	Biosensors (Basel) Vol. 11; no. 2; p. 34
Main Authors:	Moumanis, Khalid, Sirbu, Lilian, Hassen, Walid Mohamed, Frost, Eric, Carvalho, Lydston Rodrigues de, Hiernaux, Pierre, Dubowski, Jan Jerzy
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 27-01-2021 MDPI
Subjects:	Backwash Bacteria Biosensors concentration factor Contamination cooling towers Drinking water E coli Environmental monitoring Environmental Monitoring - methods Epidemics Filters Filtration filtration system Health hazards Industrial water Laboratories Legionella pneumophila Legionella pneumophila - isolation & purification Legionnaires' disease bacterium Legionnaires’ disease Microorganisms Modules Polymerase chain reaction Public health Remote monitoring Saline solutions Water Water analysis Water Microbiology Water pollution Water Pollution - statistics & numerical data Water purification water samples Water sampling Canada Legionnaires’ disease bacteria cooling towers recovery rate water samples Legionella pneumophila concentration factor filtration system
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Summary:	The detection of water contamination with is of critical importance to manufacturers of water processing equipment and public health entities dealing with water networks and distribution systems. Detection methods based on polymerase chain reaction or biosensor technologies require preconcentration steps to achieve attractive sensitivity levels. Preconcentration must also be included in protocols of automated collection of water samples by systems designed for quasi-continuous monitoring of remotely located water reservoirs for the presence of . We designed and characterized a water sampling module for filtration and backwashing intended for analysis of low-to-medium contaminated water, typically with bacteria not exceeding 50 colony-forming units per milliliter. The concentration factors of 10× and 21× were achieved with 0.22 and 0.45 µm filters, respectively, for samples of bacteria prepared in clean saline solutions. However, a 5× concentration factor was achieved with 0.45 µm filters for a heavily contaminated or turbid water typical of some industrial water samples.
ISSN:	2079-6374 2079-6374
DOI:	10.3390/BIOS11020034