vesicular stomatitis pseudovirus expressing the surface glycoproteins of influenza A virus

Pseudotyped viruses bearing the glycoprotein(s) of a donor virus over the nucleocapsid core of a surrogate virus are widely used as safe substitutes for infectious virus in virology studies. Retroviral particles pseudotyped with influenza A virus glycoproteins have been used recently for the study o...

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Bibliographic Details
Published in:	Archives of virology Vol. 159; no. 10; pp. 2651 - 2658
Main Authors:	Cheresiz, S. V, Kononova, A. A, Razumova, Yu. V, Dubich, T. S, Chepurnov, A. A, Kushch, A. A, Davey, R, Pokrovsky, A. G
Format:	Journal Article
Language:	English
Published:	Vienna Springer-Verlag 01-10-2014 Springer Vienna Springer Nature B.V
Subjects:	Antibodies, Neutralizing - immunology Antibodies, Viral - immunology Avian flu Biomedical and Life Sciences Biomedicine Chimera - genetics Genomes Glycoproteins HEK293 Cells Hemagglutinin Glycoproteins, Influenza Virus - biosynthesis Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology hemagglutinins Humans Infections Infectious Diseases influenza Influenza A virus Influenza A Virus, H5N1 Subtype - genetics Laboratories Medical Microbiology Neuraminidase - genetics Neuraminidase - immunology neutralization tests Original Article Orthomyxoviridae Oseltamivir - analogs & derivatives Oseltamivir - pharmacology pathogenicity Plasmids Proteins Pseudovirus screening sialidase vesicular stomatitis Vesicular Stomatitis - pathology Vesicular Stomatitis - virology Vesicular stomatitis Indiana virus - genetics Vesicular stomatitis Indiana virus - metabolism Vesicular stomatitis Indiana virus - pathogenicity Virology Viruses United States > US California Russia Influenza Virus Oseltamivir Carboxylate Influenza Avian Influenza 293FT Cell
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Summary:	Pseudotyped viruses bearing the glycoprotein(s) of a donor virus over the nucleocapsid core of a surrogate virus are widely used as safe substitutes for infectious virus in virology studies. Retroviral particles pseudotyped with influenza A virus glycoproteins have been used recently for the study of influenza hemagglutinin and neuraminidase-dependent processes. Here, we report the development of vesicular-stomatitis-virus-based pseudotypes bearing the glycoproteins of influenza A virus. We show that pseudotypes bearing the hemagglutinin and neuraminidase of H5N1 influenza A virus mimic the wild-type virus in neutralization assays and sensitivity to entry inhibitors. We demonstrate the requirement of NA for the infectivity of pseudotypes and show that viruses obtained with different NA proteins are significantly different in their transduction activities. Inhibition studies with oseltamivir carboxylate show that neuraminidase activity is required for pseudovirus production, but not for the infection of target cells with H5N1-VSV pseudovirus. The HA-NA-VSV pseudoviruses have high transduction titers and better stability than the previously reported retroviral pseudotypes and can replace live influenza virus in the development of neutralization assays, screening of potential antivirals, and the study of different HA/NA reassortants.
Bibliography:	http://dx.doi.org/10.1007/s00705-014-2127-y ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-014-2127-y