Localization and expression of putative circadian clock transcripts in the brain of the nudibranch Melibe leonina

Localization and expression of putative circadian clock transcripts in the brain of the nudibranch Melibe leonina

The nudibranch, Melibe leonina, expresses a circadian rhythm of locomotion, and we recently determined the sequences of multiple circadian clock transcripts that may play a role in controlling these daily patterns of behavior. In this study, we used these genomic data to help us: 1) identify putativ...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 223; pp. 52 - 59
Main Authors: Duback, Victoria E., Sabrina Pankey, M., Thomas, Rachel I., Huyck, Taylor L., Mbarani, Izhar M., Bernier, Kyle R., Cook, Geoffrey M., O'Dowd, Colleen A., Newcomb, James M., Watson, Winsor H.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-09-2018
Subjects:
Animals
Biological clock
Brain - metabolism
Circadian Clocks - genetics
Circadian rhythm
Clock genes
Cryptochrome
Gastropod
Gastropoda - genetics
Gene Expression Profiling
In Situ Hybridization, Fluorescence
Nudibranch
Polymerase Chain Reaction - methods
RNA Probes
RNA, Messenger - genetics
Biological clock
Gastropod
Circadian rhythm
Clock genes
Nudibranch
Cryptochrome
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Summary:The nudibranch, Melibe leonina, expresses a circadian rhythm of locomotion, and we recently determined the sequences of multiple circadian clock transcripts that may play a role in controlling these daily patterns of behavior. In this study, we used these genomic data to help us: 1) identify putative clock neurons using fluorescent in situ hybridization (FISH); and 2) determine if there is a daily rhythm of expression of clock transcripts in the M. leonina brain, using quantitative PCR. FISH indicated the presence of the clock-related transcripts clock, period, and photoreceptive and non-photoreceptive cryptochrome (pcry and npcry, respectively) in two bilateral neurons in each cerebropleural ganglion and a group of <10 neurons in the anterolateral region of each pedal ganglion. Double-label experiments confirmed colocalization of all four clock transcripts with each other. Quantitative PCR demonstrated that the genes clock, period, pcry and npcry exhibited significant differences in expression levels over 24 h. These data suggest that the putative circadian clock network in M. leonina consists of a small number of identifiable neurons that express circadian genes with a daily rhythm.
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ISSN:1095-6433
1531-4332
1531-4332
DOI:10.1016/j.cbpa.2018.05.002