The up-regulation of long non-coding RNA CCAT2 indicates a poor prognosis for prostate cancer and promotes metastasis by affecting epithelial-mesenchymal transition

Recently, long noncoding RNAs (lncRNAs) have been shown to have critical regulatory roles in human cancer biology. LncRNA CCAT2 is a novel identified lncRNA that was previously reported to be up-regulated in different cancers, however, its role in prostate cancer remains unclear. The aim of this stu...

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Published in:	Biochemical and biophysical research communications Vol. 480; no. 4; pp. 508 - 514
Main Authors:	Zheng, Jinfeng, Zhao, Shaoxiong, He, Xiaolong, Zheng, Zhili, Bai, Weibin, Duan, Youlin, Cheng, Shanlin, Wang, Juncai, Liu, Xucheng, Zhang, Gang
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 25-11-2016
Subjects:	60 APPLIED LIFE SCIENCES Aged Aged, 80 and over ANIMAL TISSUES BIOLOGICAL MARKERS Biomarkers, Tumor - genetics CCAT2 Cell Line, Tumor Epithelial-Mesenchymal Transition - genetics Genetic Markers - genetics Humans Incidence INDICATORS Long non-coding RNA Male METASTASES Middle Aged MIGRATION MULTIVARIATE ANALYSIS NEOPLASMS PATIENTS PLANT GROWTH PLANT TISSUES POLAR-CAP ABSORPTION POLYMERASE CHAIN REACTION Prognosis PROSTATE Prostate cancer Prostatic Neoplasms - genetics Prostatic Neoplasms - mortality Prostatic Neoplasms - secondary Risk Factors RNA RNA, Long Noncoding - genetics Survival Rate Up-Regulation - genetics CCAT2 lncRNAs Prognosis OS qRT-PCR PFS Prostate cancer EMT Long non-coding RNA PCa
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Summary:	Recently, long noncoding RNAs (lncRNAs) have been shown to have critical regulatory roles in human cancer biology. LncRNA CCAT2 is a novel identified lncRNA that was previously reported to be up-regulated in different cancers, however, its role in prostate cancer remains unclear. The aim of this study was to investigate the expression and role of lncRNA CCAT2 in prostate cancer. The expression levels of lncRNA CCAT2 in PCa tissues and cell lines (DU145 and 22RV1) were evaluated by quantitative real-time PCR (qRT-PCR), and its association with prognosis of patients was analyzed by statistical analysis. Furthermore, the effect of CCAT2 on proliferation, migration, and invasion was studied in PCa cells. We found that the expression level of CCAT2 was higher in PCa tissues and cells compared to adjacent non-tumor tissues and normal prostate stromal immortalized cells WPMY-1. Kaplan-Meier survival analysis revealed that patients with high CCAT2 expression level had poorer overall survival and progression-free survival than those with low CCAT2 expression. Furthermore, multivariate analysis showed that the status of CCAT2 expression was an independent prognostic indicator for this disease. We also found that knockdown of CCAT2 could inhibit cell growth, migration, and invasion in vitro. In addition, knockdown of CCAT2 stimulated epithelial-mesenchymal transition (EMT) through abrogating N-cadherin, vimentin expression and intensifing the expression levels of E-cadherin. In conclusion, our data suggested that lncRNA CCAT2 was a novel molecule involved in PCa progression, which provided a potential prognostic biomarker and therapeutic target for new therapies in patients with prostate cancer. •LncRNA CCAT2 is identified with overexpression in prostate cancer tissues and cell lines.•High expression of lncRNA CCAT2 can be an independent prognostic factor for overall survival and progression-free survival.•Knockdown of lncRNA CCAT2 can inhibit cell growth, migration, and invasion in vitro study.•Knockdown of lncRNA CCAT2 stimulates metastasis of prostate cancer cell through regulating EMT-associated gene expression including N-cadherin, Vimentin and E-cadherin.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1016/j.bbrc.2016.08.120