Search Results - "Du Plessis, D.H"

Nucleotide sequence and translation of satellite tobacco mosaic virus RNA by Mirkov, T E, Mathews, D M, Du Plessis, D H, Dodds, J A

“…Satellite tobacco mosaic virus (STMV) is a plant virus with a 17-nm icosahedral particle encapsidating a 0.3 X 10(6) Mr ssRNA genome that depends on…”
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An indirect sandwich ELISA utilising F(ab')2 fragments for the detection of African horsesickness virus by du Plessis, D H, van Wyngaardt, W, Bremer, C W

“…African horsesickness virus (AHSV), an important disease of equines is caused by an orbivirus. Because of the need to contain the spread of the disease, it is…”
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Serogroup-reactive and type-specific detection of bluetongue virus antibodies using chicken scFvs in inhibition ELISAs by Fehrsen, J., van Wyngaardt, W., Mashau, C., Potgieter, A.C., Chaudhary, V.K., Gupta, A., Jordaan, F.A., du Plessis, D.H.

“…Two bluetongue virus (BTV) serotype 10-specific single-chain Fv chicken antibody fragments (scFvs) were evaluated in a competitive ELISA. The binding of one…”
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Use of a gene-targeted phage display random epitope library to map an antigenic determinant on the bluetongue virus outer capsid protein VP5 by Wang, Lin-Fa, Du Plessis, Dion H., White, John R., Hyatt, Alex D., Eaton, Bryan T.

“…We describe the use of a gene-targeted random epitope library for the mapping of antigenic determinants. A DNA clone encoding the target antigen was digested…”
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Low molecular weight proteins of Cowdria ruminantium (Welgevonden isolate) induce bovine CD4 +-enriched T-cells to proliferate and produce interferon-γ by Van Kleef, M, Macmillan, H, Gunter, N.J, Zweygarth, E, Allsopp, B.A, Shkap, V, Du Plessis, D.H, Brown, W.C

“…An important objective in vaccination strategies is to activate lymphocytes with particular effector functions. Cellular immunity and the type I cytokine IFN-γ…”
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Immune responses in a horse inoculated with the VP2 gene of African horsesickness virus by Romito, M, Du Plessis, D H, Viljoen, G J

“…The ability of a DNA vaccine to elicit an immune response in a horse was evaluated. The outer capsid protein VP2 of African horsesickness virus is known to…”
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Cross-reactive epitope mimics in a fragmented-genome phage display library derived from the rickettsia, Cowdria ruminantium by Fehrsen, J, du Plessis, D H

“…Epitopes can be mapped by comparing immunoaffinity-selected peptides from fragmented-gene display libraries with the target gene. With larger libraries derived…”
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The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus by Du Plessis, D H, Van Wyngaardt, W, Romito, M, Du Plessis, M, Maree, S

“…An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension…”
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Group-reactive ELISAs for detecting antibodies to African horsesickness and equine encephalosis viruses in horse, donkey, and zebra sera by Williams, R, Du Plessis, D H, Van Wyngaardt, W

“…Group-reactive enzyme-linked immunosorbent assays (ELISAs) were developed to selectively detect antibodies to African horsesickness virus (AHSV) and equine…”
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Identification of an antigenic peptide specific for bluetongue virus using phage display expression of NSI sequences by Du Plessis, D.H., Romito, M., Jordaan, Frances

“…Background: NSI is a non-structural protein associated with the replication of bluetongue virus (BTV), an orbivirus (Reoviridae) that infects sheep and cattle…”
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Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies by Van Wyngaardt, W, Du Plessis, D H

“…A filamentous phage library displaying a vast repertoire of synthetic single chain fragment variable (scFv) antibody fragments was subjected to affinity…”
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Vaccinia virus expression of the VP7 protein of South African bluetongue virus serotype 4 and its use as an antigen in a capture ELISA by Cloete, M, Du Plessis, D.H, Van Dijk, A.A, Huismans, H, Viljoen, G.J

“…Recombinant vaccinia viruses expressing the VP7 core protein of South African bluetongue virus serotype 4 (SA-BTV4) were identified by polymerase chain…”
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Baculovirus expression of non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 and their use as antigens in an indirect ELISA by Bremer, C W, du Plessis, D H, van Dijk, A A

“…Non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 (AHSV3) were expressed in Spodoptera frugiperda cells by recombinant…”
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Serological differentiation of five bluetongue virus serotypes in indirect ELISA by Du Plessis, D H

“…The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera…”
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Cell-free translation and identification of the replicative form of Nudaurelia beta virus RNA by Du Plessis, D.H, Mokhosi, G, Hendry, D.A

“…Larvae of the pine emperor moth, Nudaurelia cytherea capensis, infected with Nudaurelia beta virus (NbetaV) consistently contained one species of dsRNA. This…”
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Production and properties of monoclonal antibodies against African horsesickness virus, serotype 3 by Van Wyngaardt, W, Du Plessis, D H, Van Wyngaardt, S, Verschoor, J A

“…Four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (McAbs) specific for African horsesickness virus (AHSV). Two…”
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Laboratory confirmation of African horsesickness in the western Cape: application of a F(ab')2-based indirect ELISA by Du Plessis, D H, Van Wyngaardt, W, Gerdes, G H, Opperman, E

“…Recently a suspected outbreak of African horsesickness in the Western Cape Province resulted in the deaths of four foals and one adult horse. Spleen samples…”
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