Search Results - "Duša, Filip"
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Fluorescein-based low-molecular-mass markers of isoelectric point for tracing pH gradient using high sensitivity capillary isoelectric focusing with laser-induced fluorescence detection
Published in Analytica chimica acta (01-12-2024)“…Successful implementation of capillary isoelectric focusing (cIEF) in general requires the use of reference points - markers of isoelectric point. The…”
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Capillary isoelectric focusing – The role of markers of isoelectric point and recent applications in the field
Published in TrAC, Trends in analytical chemistry (Regular ed.) (01-05-2023)“…Capillary isoelectric focusing (cIEF) is a very powerful separation technique. However, successful implementation of this technique in various fields of…”
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Nanoplasmonic Sensing and Capillary Electrophoresis for Fast Screening of Interactions between Phosphatidylcholine Biomembranes and Surfactants
Published in Langmuir (22-05-2018)“…Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a…”
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4
Analysis of fungi by capillary electrophoresis
Published in TrAC, Trends in analytical chemistry (Regular ed.) (01-02-2023)“…This review focuses on the capillary electrophoresis (CE) of yeasts and filamentous molds. After brief introduction and theoretical basis of the CE, it…”
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Low-molecular-mass colored compounds for fine tracing of pH gradient on broad and narrow scale in isoelectric focusing
Published in Analytica chimica acta (15-08-2022)“…We present a set of novel low-molecular-mass (LMM) compounds possessing ampholytic properties. The compounds were designed to perform as markers of isoelectric…”
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Low-molecular-mass nitrophenol-based compounds suitable for the effective tracking of pH gradient in isoelectric focusing
Published in Analytica chimica acta (17-10-2019)“…Low-molecular-mass isoelectric point (pI) markers are a reasonable alternative to commonly used peptide and protein pI markers in the field of isoelectric…”
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Effect of Ionic Liquids on Zebrafish (Danio rerio) Viability, Behavior, and Histology; Correlation between Toxicity and Ionic Liquid Aggregation
Published in Environmental science & technology (05-07-2016)“…The effect of 11 common amidinium, imidazolium, and phosphonium based ionic liquids (ILs) on zebrafish (Danio rerio) and Chinese hamster ovary cells (CHO) was…”
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Novel chip-based isoelectric focusing device for fractionation of bacteria prior to their mass spectrometry identification
Published in Analytica chimica acta (01-02-2022)“…We have developed a planar chip utilizing divergent geometry of separation channel capable of vertical free-flow electrophoresis of particles at flows of lower…”
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Effects of phosphonium-based ionic liquids on phospholipid membranes studied by small-angle X-ray scattering
Published in Chemistry and physics of lipids (01-12-2016)“…[Display omitted] •The effects of phosphonium-based ionic liquids on model membranes depend on length of hydrocarbon chains in the cation.•Ionic liquids with…”
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New solution IEF device for micropreparative separation of peptides and proteins
Published in Electrophoresis (01-06-2013)“…The article presents a new concept of preparative solution IEF where time requirements and efficiency are similar to gel‐based IEF whereas simple fraction…”
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Simple power supply for power load controlled isoelectric focusing
Published in Electrophoresis (01-04-2014)“…The power supply for IEF based on features of the Cockcroft‐Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the…”
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Monoliths in capillary electrochromatography and capillary liquid chromatography in conjunction with mass spectrometry
Published in Electrophoresis (01-04-2016)“…Here, we have reviewed separation studies utilizing monolithic capillary columns for separation of compounds preceding MS analysis. The review is divided in…”
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13
Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide
Published in Frontiers in materials (01-03-2019)“…The importance of using biomimicking membranes for various biological applications is rising, as such models are relevant for imitating real organisms. In…”
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Novel cationic polyelectrolyte coatings for capillary electrophoresis
Published in Electrophoresis (01-01-2016)“…The use of bare fused silica capillary in CE can sometimes be inconvenient due to undesirable effects including adsorption of sample or instability of the EOF…”
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15
Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance
Published in Colloids and surfaces, B, Biointerfaces (01-12-2015)“…[Display omitted] •Critical micellar concentrations of phosphonium ionic liquids were determined.•Liposomes were used as biomimicking models for investigation…”
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16
Simple automated liquid chromatographic system for splitless nano column gradient separations
Published in Journal of Chromatography A (08-02-2013)“…► We assembled a simple splitless gradient nano liquid chromatographic system. ► Capability of automated system to create various gradient profiles was…”
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Divergent-flow isoelectric focusing for separation and preparative analysis of peptides
Published in Electrophoresis (01-07-2012)“…A divergent‐flow isoelectric focusing (DF IEF) technique has been applied for the separation and preparative analysis of peptides. The parameters of the…”
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18
Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing
Published in Scientific reports (04-10-2018)“…Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T m ) of phospholipids…”
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Distribution of local anesthetics between aqueous and liposome phases
Published in Journal of Chromatography A (06-01-2017)“…•Distribution is dependent on the lipid composition and cholesterol amount.•Temperature has a minor effect on the distribution.•Thiourea is the preeminent EOF…”
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Pressurized Water Extraction as a Tool for Rapid and Efficient Isolation of Proteins from Almonds
Published in Food analytical methods (2021)“…Pressurized water extraction (PWE) is a technique suitable for efficient extraction of almond proteins, some of which are known as potent allergens. In this…”
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