Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy

Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy

Cell-based immunotherapy using natural killer (NK) cells, cytokine-induced killer (CIK) cells and dendritic cells (DCs) is emerging as a potential novel approach in the auxiliary treatment of a tumor. However, non-standard operation procedure, small-scale cell number, or human error may limit the cl...

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Bibliographic Details
Published in:Human vaccines & immunotherapeutics Vol. 14; no. 10; pp. 2516 - 2526
Main Authors: Meng, Yiming, Sun, Jing, Hu, Tingting, Ma, Yushu, Du, Tiaozhao, Kong, Cuicui, Zhang, Guirong, Yu, Tao, Piao, Haozhe
Format: Journal Article
Language:English
Published: United States Taylor & Francis 03-10-2018
Taylor & Francis Group
Subjects:
Adoptive Transfer - methods
Aged
Aged, 80 and over
Bioreactors
Cell Culture Techniques - methods
CIK cells
DCs
Dendritic Cells - immunology
Female
Humans
Immunotherapy - methods
Killer Cells, Natural - physiology
Male
Middle Aged
Neoplasms - therapy
NK cells
Research Paper
Technology, Pharmaceutical - methods
Tumor immunotherapy
WAVE bioreactor
CIK cells
NK cells
Tumor immunotherapy
DCs
WAVE bioreactor
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Summary:Cell-based immunotherapy using natural killer (NK) cells, cytokine-induced killer (CIK) cells and dendritic cells (DCs) is emerging as a potential novel approach in the auxiliary treatment of a tumor. However, non-standard operation procedure, small-scale cell number, or human error may limit the clinical development of cell-based immunotherapy. To simplify clinical scale NK cells, CIK cells and DCs expansions, we investigated the use of the WAVE bioreactor, a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes. We developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant number of cells for our adoptive cell transfer clinical protocols. The high proliferative rate, surface phenotypes, and cytotoxicity of these immune cells, as well as the safety of cultivation were analyzed to illuminate the effect of WAVE bioreactor. The results demonstrated that the benefit of utilizing modern WAVE bioreactors in cancer immunotherapy was simple, safe, and flexible production.
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ISSN:2164-5515
2164-554X
DOI:10.1080/21645515.2018.1480241