Search Results - "Drutsa, V.L."

Heterologous expression and isolation of influenza A virus nuclear export protein NEP by Golovko, A. O., Koroleva, O. N., Drutsa, V. L.

“…Influenza A virus nuclear export protein NEP (NS2, 14.4 kDa) plays a key role in various steps of the virus life cycle. Highly purified protein preparations…”
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Aggregation of Influenza A Virus Nuclear Export Protein by Golovko, A. O., Koroleva, O. N., Tolstova, A. P., Kuz’mina, N. V., Dubrovin, E. V., Drutsa, V. L.

“…Influenza A virus nuclear export protein (NEP) plays an important role in the viral life cycle. Recombinant NEP proteins containing (His) 6 -tag at either N…”
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Investigation of activity of recombinant mengovirus RNA-dependent RNA polymerase and its mutants by Shatskaya, G. S., Drutsa, V. L., Koroleva, O. N., Osterman, I. A., Dmitrieva, T. M.

“…The activities of wild-type mengovirus RNA polymerase (RdRP) and of its three mutants with C-terminal tryp-tophan residue replaced by residues of alanine…”
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Hepatitis C virus: The role of N-glycosylation sites of viral genotype 1b proteins for formation of viral particles in insect and mammalian cells by Beljelarskaya, S N, Orlova, O V, Drutsa, V L, Orlov, V A, Timohova, A V, Koroleva, N N, Popenko, V I, Ivanov, A V, Spirin, P V, Prassolov, V S, Rubtsov, P M, Kochetkov, S N

“…Hepatitis C virus (HCV) is characterized by considerable genetic variability and, as a consequence, it has 6 genotypes and multitude of subtypes. HCV envelope…”
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Purification of core enzyme of Escherichia coli RNA polymerase by affinity chromatography by Khodak, Yu. A, Koroleva, O. N, Drutsa, V. L

“…A method for isolation of a highly purified preparation of E. coli RNA polymerase core enzyme was developed based on IMPACT technology and dissociation of the…”
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Isolation and properties of human transketolase by Meshalkina, L. E, Solovjeva, O. N, Khodak, Yu. A, Drutsa, V. L, Kochetov, G. A

“…Recombinant human (His)₆-transketolase (hTK) was obtained in preparative amounts by heterologous expression of the gene encoding human transketolase in…”
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In vivo promoter activity of the synthetic Pribnow box by Koroleva, O.N., Drutsa, V.L.

“…The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA), ??? a containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a…”
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Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids by Drutsa, V.L., Kaberdin, V.R.

“…Several methods of oligonucleotide-directed mutagenesis in plasmids are currently available. Unfortunately, low frequencies of target mutations are commonly…”
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Effects of amino-terminus truncation in human cytochrome P450IID6 on its insertion into the endoplasmic reticulum membrane of Saccharomyces cerevisiae by Krynetsky, E.Yu, Drutsa, V.L., Kovaleva, I.E., Luzikov, V.N., Uvarov, V.Yu

“…A truncated form of cytochrome P450IID6 deprived of 22 NH 2-terminal amino acids residues (P450IID6Δ1-22) was found in both the cytosol and the microsomal…”
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Simple approach for oligonucleotide-directed mutagenesis of any double-stranded circular DNA by Drutsa, V.L., Kaberdin, V.R.

“…A new site-directed method for introducing mutations into any region of plasmid vector close to the unique restriction site is described. It is based on the…”
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DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp) by Shabarova, Z.A., Dolinnaya, N.G., Drutsa, V.L., Mehiikova, N.P., Purmal, A.A.

“…Self-association of a decanucleotide d(TGGCCAAGCTp) in an aqueous solution is shown by UV spectroscopy, CD and sedimentation analysis to yield a…”
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‘Half-site’ affinity modification of tryptophanyl-tRNA synthetase leads to ‘freezing’ of the free subunit by Favorova, O.O., Madoyan, I.A., Drutsa, V.L.

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active-site-directed adenosine triphosphate analogue binds to the beta-subunits of factor F1 mitochondrial adenosine triphosphatase with its triphosphate moiety by Drutsa, V.L, Kozlov, I.A, Milgrom, Y.M, Shabarova, Z.A, Sokolova, N.I

“…The reaction of the mixed anhydride of [3H]ATP and mesitylenecarboxylic acid and soluble mitochondrial adenosine triphosphatase is accompanied by the covalent…”
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Investigation of activation of phosphate groups in mono-and oligonucleotides with mesitoyl chloride by Drutsa, V.L., Zarytova, V.F., Knorre, D.G., Lebedev, A.V., Sokolova, N.I., Shabarova, Z.A.

“…It has been demonstrated with the use of 31P NMR pulsed spectroscopy that the reaction of mesitoyl chloride (MsCOCl) both with terminal and internucleotide…”
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