A conditionally lethal yeast mutant blocked at the first step in glycosyl phosphatidylinositol anchor synthesis

Glycosyl phosphatidylinositols (GPIs) anchor many proteins to the surface of eukaryotic cells and may also serve as sorting signals on proteins and participate in signal transduction. We have isolated a Saccharomyces cerevisiae GPI anchoring mutant, gpi1, using a colony screen for cells blocked in [...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 269; no. 14; pp. 10193 - 10196
Main Authors:	Leidich, S.D, Drapp, D.A, Orlean, P
Format:	Journal Article
Language:	English
Published:	Bethesda, MD American Society for Biochemistry and Molecular Biology 08-04-1994
Subjects:	AMINOAZUCARES AMINOSUCRE Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrate Sequence Crosses, Genetic FOSFOLIPIDOS Fundamental and applied biological sciences. Psychology Genes, Fungal Genes, Lethal Glycerolipids, phospholipids Glycosylphosphatidylinositols - biosynthesis Lipids LIPOGENESE LIPOGENESIS Molecular Sequence Data MUTACION INDUCIDA MUTANT MUTANTES Mutation MUTATION PROVOQUEE Other biological molecules PHOSPHATIDE PROTEINAS PROTEINE REACCIONES QUIMICAS REACTION CHIMIQUE SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Fungi Proteins Glycophospholipid Yeast Radiolabelling Molecular assembly Ascomycetes Biosynthesis Mutation Lethal character Saccharomyces cerevisiae Thallophyta
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Summary:	Glycosyl phosphatidylinositols (GPIs) anchor many proteins to the surface of eukaryotic cells and may also serve as sorting signals on proteins and participate in signal transduction. We have isolated a Saccharomyces cerevisiae GPI anchoring mutant, gpi1, using a colony screen for cells blocked in [3H]inositol incorporation into protein. The gpi1 mutant is defective in vitro in the synthesis of N-acetylglucosaminyl phosphatidylinositol, the first intermediate in GPI synthesis, and is also temperature-sensitive for growth. Completion of the first step in GPI assembly is therefore required for growth of the unicellular eukaryote S. cerevisiae. GPI synthesis could therefore be exploited as a target for antifungal or antiparasitic agents
Bibliography:	9450596 F60 F30 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9258 1083-351X
DOI:	10.1016/s0021-9258(17)34042-5