Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in Saccharomyces cerevisiae

Saccharomyces cerevisiae mutants lacking oxidative stress response genes were used to investigate which genes are required under normal aerobic conditions to maintain cellular redox homeostasis, using intracellular glutathione redox potential (glutathione E h) to indicate the redox environment of th...

Full description

Saved in:

Bibliographic Details
Published in:	FEMS yeast research Vol. 5; no. 12; pp. 1215 - 1228
Main Authors:	Drakulic, Tamara, Temple, Mark D., Guido, Ron, Jarolim, Stefanie, Breitenbach, Michael, Attfield, Paul V., Dawes, Ian W.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Elsevier B.V 01-12-2005 Blackwell Publishing Ltd Oxford University Press
Subjects:	Aerobic conditions Ageing Aging Antioxidants Catalase - genetics Catalase - metabolism Cellular Senescence Cellular stress response Detoxification DNA Fragmentation Ethidium - analogs & derivatives Flow Cytometry Fluorescent Dyes Fluorescent indicators Genes, Fungal Glutathione Glutathione - metabolism Glutathione reductase Glutathione Reductase - genetics Glutathione Reductase - metabolism Homeostasis Indoles - pharmacology Intracellular Membrane Potentials Mitochondria Mitochondria - physiology Mutants Oxidants Oxidation Oxidation-Reduction Oxidative stress Oxidative Stress - genetics Reactive oxygen species Reactive Oxygen Species - metabolism Redox potential Rhodamine Rhodamine 123 Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - physiology Staining and Labeling Stationary phase Stress response Superoxide Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Thioredoxin Thioredoxin-Disulfide Reductase - genetics Thioredoxin-Disulfide Reductase - metabolism Thioredoxins - metabolism Yeast Antioxidants Oxidative stress Redox potential Ageing Glutathione
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Saccharomyces cerevisiae mutants lacking oxidative stress response genes were used to investigate which genes are required under normal aerobic conditions to maintain cellular redox homeostasis, using intracellular glutathione redox potential (glutathione E h) to indicate the redox environment of the cells. Levels of reactive oxygen species (ROS) and mitochondrial membrane potentials (MMP) were also assessed by FACS using dihydroethidium and rhodamine 123 as fluorescent probes. Cells became more oxidised as strains shifted from exponential growth to stationary phase. During both phases the presence of reduced thioredoxin and the activity of glutathione reductase were important for redox homeostasis. Thioredoxin reductase contributed less during exponential phase when there was a strong requirement for active Yap1p transcription factor, but was critical during stationary phase. The absence of ROS detoxification systems, such as catalases or superoxide dismutases, had a lesser effect on glutathione E h, but a more pronounced effect on ROS levels and MMP. These results reflect the major shift in ROS generation as cells switch from fermentative to respiratory metabolism and also showed that there was not a strong correlation between ROS production, MMP and cellular redox environment. Heterogeneity was detected in populations of strains with compromised anti-oxidant defences, and as cells aged they shifted from one cell type with low ROS content to another with much higher intracellular ROS.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1567-1356 1567-1364
DOI:	10.1016/j.femsyr.2005.06.001