Search Results - "Drake, Penelope M"

Sweetening the Pot: Adding Glycosylation to the Biomarker Discovery Equation by Drake, Penelope M, Cho, Wonryeon, Li, Bensheng, Prakobphol, Akraporn, Johansen, Eric, Anderson, N. Leigh, Regnier, Fred E, Gibson, Bradford W, Fisher, Susan J

“…Cancer has profound effects on gene expression, including a cell's glycosylation machinery. Thus, tumors produce glycoproteins that carry oligosaccharides with…”
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Maytansine-bearing antibody-drug conjugates induce in vitro hallmarks of immunogenic cell death selectively in antigen-positive target cells by Bauzon, Maxine, Drake, Penelope M, Barfield, Robyn M, Cornali, Brandon M, Rupniewski, Igor, Rabuka, David

“…Oncology treatment has been revolutionized by the introduction of immune checkpoint inhibitor drugs, which enable 20-40% of patients to generate anti-tumor…”
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Recent Developments in ADC Technology: Preclinical Studies Signal Future Clinical Trends by Drake, Penelope M., Rabuka, David

“…The antibody–drug conjugate (ADC) field is in a transitional period. Older approaches to conjugate composition and dosing regimens still dominate the ADC…”
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Polysialic acid enhances the migration and invasion of human cytotrophoblasts by Hromatka, Bethann S, Drake, Penelope M, Kapidzic, Mirhan, Stolp, Haley, Goldfien, Gabriel A, Shih, Ie-Ming, Fisher, Susan J

“…Polysialic acid (polySia) is a large, cell-surface linear homopolymer composed of α2,8-linked sialic acid residues. Most extensively studied in the nervous…”
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A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma by Drake, Penelope M., Schilling, Birgit, Niles, Richard K., Braten, Miles, Johansen, Eric, Liu, Haichuan, Lerch, Michael, Sorensen, Dylan J., Li, Bensheng, Allen, Simon, Hall, Steven C., Witkowska, H. Ewa, Regnier, Fred E., Gibson, Bradford W., Fisher, Susan J.

“…Glycans are cell-type-specific, posttranslational protein modifications that are modulated during developmental and disease processes. As such, glycoproteins…”
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Antibody-Drug Conjugates Targeting the Urokinase Receptor (uPAR) as a Possible Treatment of Aggressive Breast Cancer by Harel, Efrat T, Drake, Penelope M, Barfield, Robyn M, Lui, Irene, Farr-Jones, Shauna, Van't Veer, Laura, Gartner, Zev J, Green, Evan M, Lourenço, André Luiz, Cheng, Yifan, Hann, Byron C, Rabuka, David, Craik, Charles S

“…A promising molecular target for aggressive cancers is the urokinase receptor (uPAR). A fully human, recombinant antibody that binds uPAR to form a stable…”
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An emerging playbook for antibody–drug conjugates: lessons from the laboratory and clinic suggest a strategy for improving efficacy and safety by Drake, Penelope M, Rabuka, David

“…•Review of current challenges facing ADC development.•Examine relationship between ADC composition and function.•Opportunities for ADC innovation and future…”
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Polysialic Acid Governs T-Cell Development by Regulating Progenitor Access to the Thymus by Drake, Penelope M., Stock, Christina M., Nathan, Jay K., Gip, Phung, Golden, Kevin P. K., Weinhold, Birgit, Gerardy-Schahn, Rita, Bertozzi, Carolyn R.

“…Although the polysialyltransferase ST8Sia IV is expressed in both primary and secondary human lymphoid organs, its product, polysialic acid (polySia), has been…”
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Tandem-Cleavage Linkers Improve the In Vivo Stability and Tolerability of Antibody–Drug Conjugates by Chuprakov, Stepan, Ogunkoya, Ayodele O, Barfield, Robyn M, Bauzon, Maxine, Hickle, Colin, Kim, Yun Cheol, Yeo, Dominick, Zhang, Fangjiu, Rabuka, David, Drake, Penelope M

“…Although peptide motifs represent the majority of cleavable linkers used in clinical-stage antibody–drug conjugates (ADCs), the sequences are often sensitive…”
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Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes by Drake, Penelope M, Albers, Aaron E, Baker, Jeanne, Banas, Stefanie, Barfield, Robyn M, Bhat, Abhijit S, de Hart, Gregory W, Garofalo, Albert W, Holder, Patrick, Jones, Lesley C, Kudirka, Romas, McFarland, Jesse, Zmolek, Wes, Rabuka, David

“…It is becoming increasingly clear that site-specific conjugation offers significant advantages over conventional conjugation chemistries used to make…”
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Human pregnancy: the role of chemokine networks at the fetal-maternal interface by Red-Horse, Kristy, Drake, Penelope M, Fisher, Susan J

“…Chemokines are multifunctional molecules initially described as having a role in leukocyte trafficking and later found to participate in developmental…”
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Reciprocal chemokine receptor and ligand expression in the human placenta: Implications for cytotrophoblast differentiation by Drake, Penelope M., Red‐Horse, Kristy, Fisher, Susan J.

“…At the onset of pregnancy, the human placenta, which forms the interface between the embryo/fetus and the mother, must rapidly develop into a life‐sustaining…”
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Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates by Agarwal, Paresh, Kudirka, Romas, Albers, Aaron E, Barfield, Robyn M, de Hart, Gregory W, Drake, Penelope M, Jones, Lesley C, Rabuka, David

“…Aldehyde- and ketone-functionalized biomolecules have found widespread use in biochemical and biotechnological fields. They are typically conjugated with…”
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A Case Study to Identify the Drug Conjugation Site of a Site-Specific Antibody-Drug-Conjugate Using Middle-Down Mass Spectrometry by Hernandez-Alba, Oscar, Houel, Stéphane, Hessmann, Steve, Erb, Stéphane, Rabuka, David, Huguet, Romain, Josephs, Jonathan, Beck, Alain, Drake, Penelope M., Cianférani, Sarah

“…Middle-down mass spectrometry (MD MS) has emerged as a promising alternative to classical bottom-up approaches for protein characterization. Middle-level…”
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IDPicker 2.0: Improved Protein Assembly with High Discrimination Peptide Identification Filtering by Ma, Ze-Qiang, Dasari, Surendra, Chambers, Matthew C., Litton, Michael D., Sobecki, Scott M., Zimmerman, Lisa J., Halvey, Patrick J., Schilling, Birgit, Drake, Penelope M., Gibson, Bradford W., Tabb, David L.

“…Tandem mass spectrometry-based shotgun proteomics has become a widespread technology for analyzing complex protein mixtures. A number of database searching…”
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Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion by Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bañas, Stefanie, de Hart, Gregory W., Baker, Jeanne, Rabuka, David

“…To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of…”
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Reversal of gene dysregulation in cultured cytotrophoblasts reveals possible causes of preeclampsia by Zhou, Yan, Gormley, Matthew J, Hunkapiller, Nathan M, Kapidzic, Mirhan, Stolyarov, Yana, Feng, Victoria, Nishida, Masakazu, Drake, Penelope M, Bianco, Katherine, Wang, Fei, McMaster, Michael T, Fisher, Susan J

“…During human pregnancy, a subset of placental cytotrophoblasts (CTBs) differentiates into cells that aggressively invade the uterus and its vasculature,…”
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A Novel HER2-targeted Antibody–drug Conjugate Offers the Possibility of Clinical Dosing at Trastuzumab-equivalent Exposure Levels by Barfield, Robyn M., Kim, Yun Cheol, Chuprakov, Stepan, Zhang, Fangjiu, Bauzon, Maxine, Ogunkoya, Ayodele O., Yeo, Dominick, Hickle, Colin, Pegram, Mark D., Rabuka, David, Drake, Penelope M.

“…Abstract Trastuzumab and the related ADC, ado-trastuzumab emtansine (T-DM1), both target HER2-overexpressing cells. Together, these drugs have treatment…”
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Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates by Botzanowski, Thomas, Erb, Stéphane, Hernandez-Alba, Oscar, Ehkirch, Anthony, Colas, Olivier, Wagner-Rousset, Elsa, Rabuka, David, Beck, Alain, Drake, Penelope M, Cianférani, Sarah

“…Antibody-drug conjugates (ADCs) have emerged as a family of compounds with promise as efficient immunotherapies. First-generation ADCs were generated mostly…”
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A simple LC/MRM–MS-based method to quantify free linker-payload in antibody-drug conjugate preparations by Zmolek, Wesley, Bañas, Stefanie, Barfield, Robyn M., Rabuka, David, Drake, Penelope M.

“…•A simple MRM-based method to quantify free linker-payload in an ADC is described.•The approach has an LLOQ of 10nM and requires no special laboratory…”
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