Search Results - "Dragan, S.M."

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA by Kostyuk, D.A., Dragan, S.M., Lyakhov, D.L., Rechinsky, V.O., Tunitskaya, V.L., Chernov, B.K., Kochetkov, S.N.

“…A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is…”
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Tyr-571 is involved in the T7 RNA polymerase binding to its promoter by Rechinsky, V.O., Tunitskaya, V.L., Dragan, S.M., Kostyuk, D.A., Kochetkov, S.N.

“…The in vitro studies of three T7 RNA polymerase point mutants suggest that substitutions of Ala and Thr for Pro-563 and of Ser for Tyr-571 have little effect…”
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Targeted mutagenesis identifies Asp-569 as a catalytically critical residue in T7 RNA polymerase by Rechinsky, V O, Chernov, B K, Dragan, S M, Kostyuk, D A, Tunitskaya, V L, Kochetkov, S N

“…In order to look more closely at a well-conserved region in T7 RNA polymerase (T7 RNAP) containing, as shown earlier, the functionally essential residues…”
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