Pseudoxanthomonas winnipegensis sp. nov., derived from human clinical materials and recovered from cystic fibrosis and other patient types in Canada, and emendation of Pseudoxanthomonas spadix Young et al . 2007

Twelve isolates recovered from 10 cystic fibrosis/other patient types and a variety of clinical sources, were referred to Canada's National Microbiology Laboratory over 7 years. These were assignable to the genus but were unidentifiable to species level. Patients included five males and five fe...

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Published in:	International journal of systematic and evolutionary microbiology Vol. 70; no. 12; pp. 6313 - 6322
Main Authors:	Bernard, Kathryn A, Vachon, Alicia, Pacheco, Ana Luisa, Burdz, Tamara, Wiebe, Deborah, Beniac, Daniel R, Hiebert, Shannon L, Booth, Tim, Doyle, Dena Annie, Lawson, Paul, Bernier, Anne-Marie
Format:	Journal Article
Language:	English
Published:	England 01-12-2020
Subjects:	Adolescent Aged Aged, 80 and over Bacterial Typing Techniques Base Composition Canada Child Child, Preschool Cystic Fibrosis - microbiology DNA, Bacterial - genetics Fatty Acids - chemistry Female Humans Infant Male Middle Aged Nucleic Acid Hybridization Phylogeny Pigmentation RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Xanthomonadaceae - classification Xanthomonadaceae - isolation & purification Canada emendation of Pseudoxanthomonas spadix winnipegensis clinical isolates cystic fibrosis patients
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Summary:	Twelve isolates recovered from 10 cystic fibrosis/other patient types and a variety of clinical sources, were referred to Canada's National Microbiology Laboratory over 7 years. These were assignable to the genus but were unidentifiable to species level. Patients included five males and five females from two geographically separated provinces, ranging in age from 2 months to 84 years. In contrast, most species described to date have been derived from water, plants or contaminated soils. By 16S rRNA gene sequencing, the patient strains had ≥99.4 % similarity to each other but only 97.73-98.29 % to their closest relatives, or . Bacteria were studied by whole genome sequencing using average nucleotide identity by Blastn, digital DNA-DNA hybridization, average amino acid identity, core genome and single nucleotide variant analyses, MALDI-TOF, biochemical and cellular fatty acid analyses, and by antimicrobial susceptibility testing. Bacterial structures were assessed using scanning and transmission electron microscopy. Strains were strict aerobes, yellowish-pigmented, oxidative, non-motile, Gram-stain-negative bacilli and generally unable to reduce nitrate. Strains were susceptible to most of the antibiotics tested; some resistance was observed towards carbapenems, several cephems and uniformly to nitrofurantoin. The single taxon group observed by 16S rRNA gene sequencing was supported by whole genome sequencing; genomes ranged in size from 4.36 to 4.73 Mb and had an average G+C content of 69.12 mol%. Based on this study we propose the name sp. nov. for this cluster. DSM 18855 , acquired for this study, was found to be non-motile phenotypically and by electron microscopy; we therefore propose the emendation of Young . 2007 to document that observation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1466-5026 1466-5034
DOI:	10.1099/ijsem.0.004533