Search Results - "Downey, Christopher D."

Chaperoning of a Replicative Polymerase onto a Newly Assembled DNA-Bound Sliding Clamp by the Clamp Loader by Downey, Christopher D., McHenry, Charles S.

“…Cellular replicases contain multiprotein ATPases that load sliding clamp processivity factors onto DNA. We reveal an additional role for the DnaX clamp loader:…”
Get full text

The Rate of Polymerase Release upon Filling the Gap between Okazaki Fragments Is Inadequate to Support Cycling during Lagging Strand Synthesis by Dohrmann, Paul R., Manhart, Carol M., Downey, Christopher D., McHenry, Charles S.

“…Upon completion of synthesis of an Okazaki fragment, the lagging strand replicase must recycle to the next primer at the replication fork in under 0.1 s to…”
Get full text

Docking Kinetics and Equilibrium of a GAAA Tetraloop-Receptor Motif Probed by Single-Molecule FRET by Hodak, Jose H., Fiore, Julie L., Nesbitt, David J., Downey, Christopher D., Pardi, Arthur, Uhlenbeck, Olke C.

“…Docking kinetics and equilibrium of fluorescently labeled RNA molecules are studied with single-molecule FRET methods. Time-resolved FRET is used to monitor…”
Get full text

Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli by Fingland, Nicholas, Flåtten, Ingvild, Downey, Christopher D., Fossum‐Raunehaug, Solveig, Skarstad, Kirsten, Crooke, Elliott

“…In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of…”
Get full text

Influence of Hydrostatic Pressure and Cosolutes on RNA Tertiary Structure by Downey, Christopher D, Crisman, Ryan L, Randolph, Theodore W, Pardi, Arthur

“…RNAs fold into complex three-dimensional structures that are critical to their various biological functions. These structures often form independently of the…”
Get full text

Polymerase Chaperoning and Multiple ATPase Sites Enable the E. coli DNA Polymerase III Holoenzyme to Rapidly Form Initiation Complexes by Downey, Christopher D., Crooke, Elliott, McHenry, Charles S.

“…Cellular replicases include three subassemblies: a DNA polymerase, a sliding clamp processivity factor, and a clamp loader complex. The Escherichia coli clamp…”
Get full text

Monovalent and Divalent Promoted GAAA Tetraloop-Receptor Tertiary Interactions from Freely Diffusing Single-Molecule Studies by Fiore, Julie L., Hodak, Jose H., Piestert, Oliver, Downey, Christopher D., Nesbitt, David J.

“…Proper assembly of RNA into catalytically active three-dimensional structures requires multiple tertiary binding interactions, individual characterization of…”
Get full text

Only One ATP-binding DnaX Subunit Is Required for Initiation Complex Formation by the Escherichia coli DNA Polymerase III Holoenzyme[S] by Wieczorek, Anna, Downey, Christopher D., Dallmann, H. Garry, McHenry, Charles S.

“…The DnaX complex (DnaX3δδ′χψ) within the Escherichia coli DNA polymerase III holoenzyme serves to load the dimeric sliding clamp processivity factor, β2, onto…”
Get full text

Metal Ion Dependence, Thermodynamics, and Kinetics for Intramolecular Docking of a GAAA Tetraloop and Receptor Connected by a Flexible Linker by Downey, Christopher D, Fiore, Julie L, Stoddard, Colby D, Hodak, Jose H, Nesbitt, David J, Pardi, Arthur

“…The GAAA tetraloop−receptor motif is a commonly occurring tertiary interaction in RNA. This motif usually occurs in combination with other tertiary…”
Get full text

Depletion of acidic phospholipids influences chromosomal replication in E scherichia coli by Fingland, Nicholas, Flåtten, Ingvild, Downey, Christopher D., Fossum‐Raunehaug, Solveig, Skarstad, Kirsten, Crooke, Elliott

“…Abstract In E scherichia coli , coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the…”
Get full text