Search Results - "Dovichi, Norman"

Third-Generation Electrokinetically Pumped Sheath-Flow Nanospray Interface with Improved Stability and Sensitivity for Automated Capillary Zone Electrophoresis–Mass Spectrometry Analysis of Complex Proteome Digests by Sun, Liangliang, Zhu, Guijie, Zhang, Zhenbin, Mou, Si, Dovichi, Norman J

“…We have reported a set of electrokinetically pumped sheath flow nanoelectrospray interfaces to couple capillary zone electrophoresis with mass spectrometry. A…”
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Thermally-initiated free radical polymerization for reproducible production of stable linear polyacrylamide coated capillaries, and their application to proteomic analysis using capillary zone electrophoresis–mass spectrometry by Zhu, Guijie, Sun, Liangliang, Dovichi, Norman J.

“…Proteomic analysis using capillary zone electrophoresis (CZE) typically is performed with linear polyacrylamide (LPA) coated capillaries. These capillaries…”
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Seamlessly Integrated Miniaturized Filter-Aided Sample Preparation Method to Fractionation Techniques for Fast, Loss-Less, and In-Depth Proteomics Analysis of 1 μg of Cell Lysates at Low Cost by Zhang, Zhenbin, Dovichi, Norman J.

“…We report an integrated platform that enabled a seamlessly coupling miniaturized filter-aided sample preparation (MICROFASP) method to high-pH reversed phase…”
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Ultrasensitive and Fast Bottom-up Analysis of Femtogram Amounts of Complex Proteome Digests by Sun, Liangliang, Zhu, Guijie, Zhao, Yimeng, Yan, Xiaojing, Mou, Si, Dovichi, Norman J.

“…Femtogram proteomics: An ultrasensitive capillary zone electrophoresis–mass spectrometry system that is based on an improved nanospray interface has been…”
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Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos by Zhang, Zhenbin, Dubiak, Kyle M, Huber, Paul W, Dovichi, Norman J

“…We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a…”
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Optimization of mass spectrometric parameters improve the identification performance of capillary zone electrophoresis for single-shot bottom-up proteomics analysis by Zhang, Zhenbin, Dovichi, Norman J.

“…The effects of MS1 injection time, MS2 injection time, dynamic exclusion time, intensity threshold, and isolation width were investigated on the numbers of…”
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Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry by Qu, Yanyan, Sun, Liangliang, Zhang, Zhenbin, Dovichi, Norman J

“…Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition. In contrast, capillary zone electrophoresis…”
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Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis by Wojcik, Roza, Dada, Oluwatosin O., Sadilek, Martin, Dovichi, Norman J.

“…We report a simple nanospray sheath‐flow interface for capillary electrophoresis. This interface relies on electrokinetic flow to drive both the separation and…”
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Single-Shot Proteomics Using Capillary Zone Electrophoresis–Electrospray Ionization-Tandem Mass Spectrometry with Production of More than 1 250 Escherichia coli Peptide Identifications in a 50 min Separation by Zhu, Guijie, Sun, Liangliang, Yan, Xiaojing, Dovichi, Norman J

“…Capillary zone electrophoresis (CZE)–electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was optimized and applied for analysis of 1–100 ng…”
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Integrated Capillary Zone Electrophoresis–Electrospray Ionization Tandem Mass Spectrometry System with an Immobilized Trypsin Microreactor for Online Digestion and Analysis of Picogram Amounts of RAW 264.7 Cell Lysate by Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J

“…A capillary zone electrophoresis (CZE) electrospray ionization (ESI) tandem mass spectrometry (MS/MS) system was integrated with an immobilized trypsin…”
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Coupling Capillary Zone Electrophoresis to a Q Exactive HF Mass Spectrometer for Top-down Proteomics: 580 Proteoform Identifications from Yeast by Zhao, Yimeng, Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J

“…We used reversed-phase liquid chromatography to separate the yeast proteome into 23 fractions. These fractions were then analyzed using capillary zone…”
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Detachable Strong Cation Exchange Monolith, Integrated with Capillary Zone Electrophoresis and Coupled with pH Gradient Elution, Produces Improved Sensitivity and Numbers of Peptide Identifications during Bottom-up Analysis of Complex Proteomes by Zhang, Zhenbin, Yan, Xiaojing, Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J

“…A detachable sulfonate-silica hybrid strong cation-exchange monolith was synthesized in a fused silica capillary, and used for solid phase extraction with…”
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Minimal deuterium isotope effects in quantitation of dimethyl‐labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry by Yan, Xiaojing, Sun, Liangliang, Dovichi, Norman J., Champion, Matthew M.

“…Stable heavy‐isotope labeling is commonly used in quantitative proteomics. Several common techniques incorporate deuterium (2H) as the heavy isotopic label…”
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Dynamic pH junction preconcentration in capillary electrophoresis- electrospray ionization-mass spectrometry for proteomics analysis by Zhu, Guijie, Sun, Liangliang, Dovichi, Norman J

“…Capillary zone electrophoresis (CZE)-electrospray ionization (ESI)-mass spectrometry (MS) is an interesting complimentary technique to reversed phase liquid…”
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Detection of 1 zmol injection of angiotensin using capillary zone electrophoresis coupled to a Q-Exactive HF mass spectrometer with an electrokinetically pumped sheath-flow electrospray interface by Amenson-Lamar, Emily Ann, Sun, Liangliang, Zhang, Zhenbin, Bohn, Paul W., Dovichi, Norman J.

“…An electrokinetically pumped sheath-flow nanoelectrospray interface provides an efficient means of transferring ions from a capillary electrophoresis system…”
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Comparison of the LTQ-Orbitrap Velos and the Q-Exactive for proteomic analysis of 1-1000 ng RAW 264.7 cell lysate digests by Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J.

“…RATIONALE There is interest in extending bottom‐up proteomics to the smallest possible sample size. We investigated the performance of two modern mass…”
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Bottom-Up Proteomics of Escherichia coli Using Dynamic pH Junction Preconcentration and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry by Zhu, Guijie, Sun, Liangliang, Yan, Xiaojing, Dovichi, Norman J

“…We report the use of the dynamic pH junction based capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) for…”
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Multisegment injections improve peptide identification rates in capillary zone electrophoresis-based bottom-up proteomics by Boley, Danielle A., Zhang, Zhenbin, Dovichi, Norman J.

“…•Multisegment injection was applied to bottom-up analysis of the yeast proteome.•Reducing time between injections to 30min resulted in a doubling of the…”
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Predicting Electrophoretic Mobility of Tryptic Peptides for High-Throughput CZE-MS Analysis by Krokhin, Oleg V, Anderson, Geoffrey, Spicer, Vic, Sun, Liangliang, Dovichi, Norman J

“…A multiparametric sequence-specific model for predicting peptide electrophoretic mobility has been developed using large-scale bottom-up proteomic CE-MS data…”
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Dual roles for ATP in the regulation of phase separated protein aggregates in Xenopus oocyte nucleoli by Hayes, Michael H, Peuchen, Elizabeth H, Dovichi, Norman J, Weeks, Daniel L

“…For many proteins, aggregation is one part of a structural equilibrium that can occur. Balancing productive aggregation versus pathogenic aggregation that…”
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